{"id":"W2319653676","doi":"10.1021/bi300298v","title":"Type IV Secretion System Core Component VirB8 from <i>Brucella</i> Binds to the Globular Domain of VirB5 and to a Periplasmic Domain of VirB6","year":2012,"lang":"en","type":"article","venue":"Biochemistry","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; McMaster University; Université de Montréal","funders":"Canadian Institutes of Health Research","keywords":"Periplasmic space; Förster resonance energy transfer; Phage display; Secretion; Function (biology); Protein–protein interaction; Cell biology; Computational biology; Domain (mathematical analysis); Biology; Chemistry; Biophysics; Peptide; Biochemistry; Physics; Gene; Escherichia coli; Fluorescence","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008901699,0.0003344695,0.0002366339,0.0001617243,0.000234536,0.0003842259,0.0001578392,0.0002706339,0.001649036],"category_scores_gemma":[0.0002005443,0.0001227948,0.0002677408,0.0001567273,0.0001627272,0.0001571988,0.0002489931,0.0003807874,0.0007782024],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005275782,"about_ca_system_score_gemma":0.0001973933,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001892979,"about_ca_topic_score_gemma":0.00171544,"domain_scores_codex":[0.9998795,0.00002159287,0.000007668962,0.00002532731,0.00004210602,0.00002393968],"domain_scores_gemma":[0.9999357,0.00001418943,0.00001312759,0.000008230107,0.000009834415,0.00001882299],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003670267,0.000008046102,0.0005642044,0.00001538083,0.000003081594,0.00004735829,0.000011777,0.00003998208,0.9985429,0.00006655561,0.00003821595,0.0006258239],"study_design_scores_gemma":[0.00001170948,0.00008467532,0.014594,0.00001368072,0.00001409293,0.0005273934,0.00007291509,0.001914085,0.9792889,0.000112199,0.00335871,0.00000763752],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994772,0.0005935277,0.002431203,0.0001322007,0.00001271194,0.000008328574,0.0002463169,0.00004235423,0.001761331],"genre_scores_gemma":[0.9900427,0.0002908507,0.005146307,0.0001179839,0.00000670608,0.00001317999,0.001698039,0.000032903,0.002651264],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001892979,"threshold_uncertainty_score":0.005516529,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006972051864233181,"score_gpt":0.2109042602814785,"score_spread":0.2039322084172453,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}