{"id":"W2320361243","doi":"10.1385/mb:14:1:25","title":"The Use of Site-Directed Mutagenesis, Transient Transfection, and Radioligand Binding; A Method for the Characterization of Receptor-Ligand Interactions","year":2000,"lang":"en","type":"article","venue":"Molecular Biotechnology","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Radioligand; Mutagenesis; Transfection; Computational biology; Site-directed mutagenesis; Ligand (biochemistry); Radioligand Assay; Biology; Transient (computer programming); Receptor; Cell biology; Chemistry; Genetics; Mutation; Mutant; Gene; Computer science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002398118,0.0001352098,0.0001742023,0.00006520077,0.0001737714,0.00001773188,0.0001477822,0.0002475046,0.00001151798],"category_scores_gemma":[0.00008802812,0.00009305211,0.0001119492,0.0001818237,0.0001749936,0.000005256428,0.00001698266,0.00007194289,6.863925e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000575953,"about_ca_system_score_gemma":0.00002225389,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002784109,"about_ca_topic_score_gemma":0.00002618102,"domain_scores_codex":[0.9991133,0.0001237311,0.0002727301,0.0002566249,0.00007082774,0.0001628504],"domain_scores_gemma":[0.9993549,0.00009302353,0.0001054809,0.0003487356,0.00007371118,0.00002412095],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002092002,0.000027313,0.000009468103,0.0000144783,0.0001200375,2.697513e-7,0.00004988772,0.00002881539,0.9100298,0.0001857959,0.00003181701,0.08929314],"study_design_scores_gemma":[0.000282351,0.0002385612,0.0001090285,0.00001389951,0.00006697509,0.00002182574,0.00001744707,0.00034581,0.8907659,0.00002794569,0.1080217,0.00008851886],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7799501,0.0004987283,0.217684,0.001163328,0.00007958142,0.0005217375,0.00007834721,0.00001986322,0.000004298503],"genre_scores_gemma":[0.9861871,0.004299369,0.00875566,0.00009585034,0.00003389059,0.0001729932,0.0001058119,0.00003653417,0.0003127942],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2089283,"threshold_uncertainty_score":0.3794554,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01157332713056087,"score_gpt":0.2415259838510941,"score_spread":0.2299526567205332,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}