{"id":"W2321572025","doi":"10.1021/bi500905b","title":"A New General Model for Predicting Melting Thermodynamics of Complementary and Mismatched B-Form Duplexes Containing Locked Nucleic Acids: Application to Probe Design for Digital PCR Detection of Somatic Mutations","year":2015,"lang":"en","type":"article","venue":"Biochemistry","topic":"DNA and Nucleic Acid Chemistry","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"Genome British Columbia; Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs","keywords":"Oligonucleotide; Duplex (building); DNA; Base pair; Locked nucleic acid; Chemistry; Digital polymerase chain reaction; Nucleic acid; Gibbs free energy; Thermodynamics; Complementary sequences; Biological system; Polymerase chain reaction; Physics; Biology; Biochemistry; Mathematics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007741097,0.000859744,0.0009347724,0.0005000827,0.0003971917,0.0006066802,0.001602665,0.001631511,0.001152426],"category_scores_gemma":[0.001969077,0.0006615209,0.001082025,0.0004833613,0.0005720494,0.001307336,0.0003724088,0.0008341323,0.0006266324],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001044202,"about_ca_system_score_gemma":0.001044229,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.009124849,"about_ca_topic_score_gemma":0.00668862,"domain_scores_codex":[0.9996483,0.00008193394,0.00001715739,0.000126966,0.00009093978,0.00003471237],"domain_scores_gemma":[0.9994855,0.0003309031,0.00005123043,0.00003046404,0.00008022348,0.00002165636],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"simulation_or_modeling","study_design_scores_codex":[0.00001922565,0.0000216482,0.0004306156,0.00003676269,0.00001522013,0.00002860224,0.00002239741,0.9870357,0.005392661,0.002905208,0.000154404,0.003937546],"study_design_scores_gemma":[0.000002258451,0.000009048608,0.00008668486,0.000001330957,0.000002979297,0.0000106393,0.000001591832,0.9979796,0.0006460039,0.001060032,0.0001957134,0.000004184403],"study_design_candidate":"simulation_or_modeling","study_design_consensus":"simulation_or_modeling","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.03934742,0.0003058,0.957813,0.0001885334,0.00004269313,0.00005436653,0.0001882517,0.0003414052,0.001718364],"genre_scores_gemma":[0.8124532,0.001096855,0.1695666,0.0002773217,0.0001103268,0.0006922249,0.0007002024,0.0003158462,0.01478744],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.009124849,"threshold_uncertainty_score":0.01814348,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02041462959417839,"score_gpt":0.2565543700131544,"score_spread":0.236139740418976,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}