{"id":"W2321642153","doi":"10.1021/sb500280q","title":"Targeted Capture and Heterologous Expression of the <i>Pseudoalteromonas</i> Alterochromide Gene Cluster in <i>Escherichia coli</i> Represents a Promising Natural Product Exploratory Platform","year":2014,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":114,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Environmental Health Sciences; Division of Ocean Sciences; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Pseudoalteromonas; Synthetic biology; Biology; Gene cluster; Heterologous expression; Gene; Computational biology; Natural product; Transformation (genetics); Function (biology); Genetics; Biochemistry; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004510108,0.0002775638,0.0005752536,0.00009489849,0.00008913315,0.0000116989,0.0002430659,0.0002500206,0.0000122777],"category_scores_gemma":[0.0006606653,0.000157905,0.0000871552,0.0001911127,0.0003878651,0.00008735084,0.00028873,0.0005010428,0.00000300852],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004218272,"about_ca_system_score_gemma":0.00004681657,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001006988,"about_ca_topic_score_gemma":0.0000506511,"domain_scores_codex":[0.9980131,0.0003114493,0.0004855009,0.0006543857,0.0001333857,0.0004022014],"domain_scores_gemma":[0.9987767,0.0001331179,0.000247136,0.0006647024,0.00009932429,0.00007903609],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000444606,0.0001171336,0.002430886,0.0001166893,0.00002503908,0.000003384545,0.0004023343,0.000002506965,0.994731,0.000008628266,0.0003430615,0.001374723],"study_design_scores_gemma":[0.00104345,0.0001619649,0.005970265,0.0002572032,0.00005146944,0.0001318811,0.00004375913,0.00008998826,0.9906712,0.0001494223,0.001248704,0.0001807399],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9947979,0.001260381,0.00001083688,0.002563062,0.0004426253,0.0008271984,0.00001873483,0.00003157519,0.00004772823],"genre_scores_gemma":[0.9972122,0.00007965996,0.0006375266,0.001754513,0.0001679327,0.000008609866,0.00002555032,0.00002862422,0.0000853308],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004059855,"threshold_uncertainty_score":0.6439181,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01094716859144393,"score_gpt":0.2299607988636701,"score_spread":0.2190136302722261,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}