{"id":"W2321642153","doi":"10.1021/sb500280q","title":"Targeted Capture and Heterologous Expression of the <i>Pseudoalteromonas</i> Alterochromide Gene Cluster in <i>Escherichia coli</i> Represents a Promising Natural Product Exploratory Platform","year":2014,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":114,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Environmental Health Sciences; Division of Ocean Sciences; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Pseudoalteromonas; Synthetic biology; Biology; Gene cluster; Heterologous expression; Gene; Computational biology; Natural product; Transformation (genetics); Function (biology); Genetics; Biochemistry; Recombinant DNA","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001364906,0.0002679611,0.0001727525,0.000116774,0.0001048898,0.0002393222,0.0001704945,0.0001675281,0.0003749439],"category_scores_gemma":[0.000115463,0.0001091093,0.0002255776,0.0001210619,0.0001884101,0.0001630093,0.0003218834,0.0003891964,0.0002389758],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002425198,"about_ca_system_score_gemma":0.0002350376,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005243739,"about_ca_topic_score_gemma":0.0008790642,"domain_scores_codex":[0.9998938,0.00001645986,0.000006982169,0.00002151778,0.00003923133,0.00002203051],"domain_scores_gemma":[0.9999188,0.00001534244,0.00003178058,0.00001302707,0.000009080997,0.00001195671],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001146914,0.00000652575,0.00009891236,0.000009509427,0.000001231022,0.00001685938,0.000005776451,0.0000405855,0.9992363,0.00009470742,0.000009343374,0.0004688615],"study_design_scores_gemma":[0.00000230309,0.0000641591,0.0006880831,0.000001569879,0.000003477204,0.00008450484,0.00001964629,0.0003357488,0.9977115,0.00004063789,0.001045869,0.000002579058],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9584218,0.0003194337,0.03847738,0.0001910156,0.00002485178,0.00008474197,0.0005364857,0.0002199759,0.001724198],"genre_scores_gemma":[0.9710651,0.0004962799,0.02591483,0.00006661688,0.000007253804,0.00004575618,0.0007507763,0.00004890384,0.001604417],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005243739,"threshold_uncertainty_score":0.001759589,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01094716859144393,"score_gpt":0.2299607988636701,"score_spread":0.2190136302722261,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}