{"id":"W2326126796","doi":"10.1021/ja512880g","title":"Peptide/Protein Stapling and Unstapling: Introduction of <i>s</i> -Tetrazine, Photochemical Release, and Regeneration of the Peptide/Protein","year":2015,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":187,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of General Medical Sciences; Beef Cattle Research Council; National Institutes of Health; National Science Foundation","keywords":"Tetrazine; Chemistry; Peptide; Thioredoxin; Bioorthogonal chemistry; Cysteine; Regeneration (biology); Biochemistry; Combinatorial chemistry; Cell biology; Organic chemistry; Click chemistry; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002929301,0.0004751909,0.0002663105,0.000190968,0.0001676505,0.0002741866,0.0003620281,0.0003433442,0.00103908],"category_scores_gemma":[0.0002423269,0.0002519892,0.0002303801,0.0001939657,0.0004340982,0.0003657727,0.000405562,0.0007263885,0.0004403633],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000286628,"about_ca_system_score_gemma":0.0003288293,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003592558,"about_ca_topic_score_gemma":0.0007912121,"domain_scores_codex":[0.9997832,0.00002214779,0.00002344382,0.00006590427,0.00006824094,0.00003699257],"domain_scores_gemma":[0.9998221,0.00002484392,0.00007828137,0.00002923446,0.00002368357,0.00002179905],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003268981,0.0000114986,0.00003455156,0.00003756278,0.000004068404,0.00004007852,0.00002191339,0.00004765947,0.9978282,0.0002938502,0.00005955985,0.001588401],"study_design_scores_gemma":[0.00000381543,0.00006613874,0.0001564709,0.000001009722,0.000002712127,0.00007132701,0.000003044445,0.0001398694,0.9986644,0.00001968864,0.0008683146,0.000003184684],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9343902,0.001206964,0.05721354,0.0001597022,0.00008044919,0.0004073708,0.0003953081,0.0006824865,0.005463947],"genre_scores_gemma":[0.9510929,0.001506647,0.03931198,0.0001625292,0.00002330157,0.0003625243,0.0006468312,0.0001708094,0.006722418],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00103908,"threshold_uncertainty_score":0.003476083,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01295401462684275,"score_gpt":0.2402837996032213,"score_spread":0.2273297849763786,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}