{"id":"W2327903390","doi":"10.1038/protex.2014.011","title":"A highly-sensitive sandwich ELISA procedure based on one-step antibody immobilization for potential in vitro diagnostics","year":2014,"lang":"en","type":"article","venue":"Protocol Exchange","topic":"Advanced Biosensing Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"In vitro; Antibody; Chemistry; Chromatography; Immunology; Medicine; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001541816,0.0001615889,0.0001432034,0.00006108991,0.00008302515,0.00001771445,0.00009311373,0.0001651849,0.000002009049],"category_scores_gemma":[0.0001138017,0.0001575521,0.00006071868,0.0001128681,0.00004253186,0.000003461966,0.00004256269,0.00006996407,0.000002190215],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002434388,"about_ca_system_score_gemma":0.00003188422,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001108895,"about_ca_topic_score_gemma":0.00001536216,"domain_scores_codex":[0.9990447,0.00004416754,0.0001863118,0.0003938453,0.0001038558,0.000227086],"domain_scores_gemma":[0.9993991,0.00003013528,0.0001073508,0.0002887327,0.0001265193,0.00004820357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005959244,0.00032853,0.00006602659,0.0001495804,0.000005295182,6.301024e-7,0.00001035771,0.0001411214,0.9915901,0.0000948594,0.0007535804,0.006263991],"study_design_scores_gemma":[0.001537134,0.000663657,0.000387925,0.00007577844,0.00001118314,0.000001751459,0.000005722007,0.02905038,0.9210091,0.0002625125,0.04674992,0.0002449804],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.005485727,0.000008055727,0.8387237,0.0004944491,0.00003226363,0.154823,0.00007845667,0.00007363296,0.0002807332],"genre_scores_gemma":[0.6835924,0.000008058028,0.03091049,0.0007770209,0.000372881,0.2836021,0.0006327005,0.00004334913,0.00006099161],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.8078132,"threshold_uncertainty_score":0.6424789,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01133954167037624,"score_gpt":0.3117314128363371,"score_spread":0.3003918711659608,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}