{"id":"W2327903390","doi":"10.1038/protex.2014.011","title":"A highly-sensitive sandwich ELISA procedure based on one-step antibody immobilization for potential in vitro diagnostics","year":2014,"lang":"en","type":"article","venue":"Protocol Exchange","topic":"Advanced Biosensing Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"In vitro; Antibody; Chemistry; Chromatography; Immunology; Medicine; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001200578,0.001559697,0.0009101232,0.0005570353,0.0002614881,0.0003638297,0.001192086,0.001106295,0.001028681],"category_scores_gemma":[0.0007143592,0.0006238923,0.000783777,0.0002990841,0.0003027343,0.0004411604,0.0006535156,0.001241334,0.001044935],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002725007,"about_ca_system_score_gemma":0.0003446609,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002334711,"about_ca_topic_score_gemma":0.0004448127,"domain_scores_codex":[0.9988247,0.0003440354,0.00009444801,0.0002263863,0.000395313,0.0001151412],"domain_scores_gemma":[0.9994724,0.0002163355,0.0000659103,0.00006003377,0.0001468618,0.00003835791],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002982331,0.00002078095,0.00006074516,0.00009321621,0.000008620657,0.00003040972,0.00001326196,0.00004857111,0.9966004,0.00007899584,0.0000550472,0.002960068],"study_design_scores_gemma":[0.000009183237,0.0002500832,0.0004675523,0.00001040948,0.00002330835,0.0003418047,0.00000806052,0.00180284,0.9949587,0.00005825378,0.002053101,0.00001675336],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2300837,0.01259171,0.7509541,0.0003368005,0.0006184527,0.0008360716,0.0005669523,0.001659761,0.002352508],"genre_scores_gemma":[0.4483954,0.006344686,0.535867,0.0004251463,0.0001712613,0.001405037,0.001188196,0.00006967247,0.006133541],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001559697,"threshold_uncertainty_score":0.006349325,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01133954167037624,"score_gpt":0.3117314128363371,"score_spread":0.3003918711659608,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}