{"id":"W2329142534","doi":"10.1101/pdb.prot080226","title":"Fluorescent Visualization of Germline Apoptosis in Living <i>Caenorhabditis elegans</i>","year":2014,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Hospital for Sick Children","funders":"","keywords":"Caenorhabditis elegans; Apoptosis; Green fluorescent protein; Acridine orange; Cell biology; Biology; RNA interference; Fluorescence; Fluorescence microscope; Programmed cell death; High-content screening; Cell; Molecular biology; Gene; RNA; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004597739,0.0001813614,0.0002352994,0.0000835215,0.00005076748,0.00003447168,0.0002678388,0.0001514031,0.00001670452],"category_scores_gemma":[0.0002267596,0.0002040065,0.00007479374,0.0001287736,0.00005752745,0.000006742068,0.000142192,0.00009000393,0.000005776979],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000253277,"about_ca_system_score_gemma":0.00005604243,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005781895,"about_ca_topic_score_gemma":0.00003870191,"domain_scores_codex":[0.9986326,0.0001108984,0.0003997194,0.0003713947,0.00019237,0.0002930472],"domain_scores_gemma":[0.9991463,0.0000206166,0.000167881,0.0004716976,0.0001148971,0.00007858009],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002078125,0.0001774429,0.03170129,0.000186809,0.00001357832,3.819933e-7,0.00007588668,0.0003916159,0.9657183,0.001402374,0.0001046697,0.0002069312],"study_design_scores_gemma":[0.000544047,0.0002888388,0.02948547,0.0002473352,0.000009908654,2.802284e-7,0.000005175518,0.0009440005,0.9545099,0.00001411634,0.0137119,0.0002390137],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9278953,0.00008867912,0.05411806,0.00005708507,0.0001193516,0.01749475,0.000007636346,0.00004120397,0.0001779195],"genre_scores_gemma":[0.9908392,0.00002506519,0.001979961,0.0001464895,0.0002153509,0.006697454,0.000003866594,0.00004635227,0.00004624493],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0629439,"threshold_uncertainty_score":0.8319142,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00912868035645164,"score_gpt":0.2543287361151239,"score_spread":0.2452000557586723,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}