{"id":"W2330098631","doi":"10.1021/ac301169q","title":"Microwave-Assisted Protein Solubilization for Mass Spectrometry-Based Shotgun Proteome Analysis","year":2012,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":27,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Chemistry; Chromatography; Mass spectrometry; Proteome; Reagent; Homogenization (climate); Shotgun proteomics; Sample preparation; Trypsin; Tandem mass spectrometry; Solubilization; Proteomics; Biochemistry; Organic chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001411711,0.001414809,0.001509769,0.001464169,0.000505569,0.0008735029,0.00124413,0.001241929,0.001690803],"category_scores_gemma":[0.001238125,0.0006614099,0.001168367,0.001655395,0.0004768655,0.001247609,0.00100602,0.002143773,0.002615743],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000460459,"about_ca_system_score_gemma":0.0004361914,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004153643,"about_ca_topic_score_gemma":0.0008289934,"domain_scores_codex":[0.9990221,0.000188421,0.000104863,0.0002321666,0.0003739113,0.0000784009],"domain_scores_gemma":[0.9995041,0.0001506274,0.0001107908,0.00006797511,0.0001287636,0.00003773965],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005284145,0.00004020978,0.0001906392,0.0004697247,0.00004353668,0.00008790015,0.00003734267,0.000152996,0.987427,0.0003220483,0.0007240381,0.01045172],"study_design_scores_gemma":[0.00002281111,0.0002548762,0.002589828,0.00007478204,0.00007855018,0.0007487871,0.00006094924,0.009781726,0.9565391,0.000843344,0.02895213,0.0000530186],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.12268,0.01857372,0.8430647,0.000594555,0.0008349062,0.00111008,0.004177771,0.005395011,0.003569314],"genre_scores_gemma":[0.1253276,0.02333697,0.8345963,0.0005524686,0.0002378203,0.001883681,0.009597472,0.0008394279,0.003628262],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001690803,"threshold_uncertainty_score":0.007465899,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02036062403464385,"score_gpt":0.2909229218933418,"score_spread":0.270562297858698,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}