{"id":"W2330266192","doi":"10.1021/bi501054b","title":"Increasing Efficiency in Protein–Protein Coupling: Subunit-Directed Acetylation and Phase-Directed CuAAC (“Click Coupling”) in the Formation of Hemoglobin Bis-Tetramers","year":2014,"lang":"en","type":"article","venue":"Biochemistry","topic":"Hemoglobin structure and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Health Canada; University of Toronto; Canadian Blood Services","keywords":"Azide; Tetramer; Chemistry; Protein subunit; Pentamer; Acetylation; Combinatorial chemistry; Allosteric regulation; Stereochemistry; Hemoglobin; Biophysics; Biochemistry; Organic chemistry; Receptor; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007299193,0.0002111305,0.0002093508,0.00008714331,0.00007502735,0.00003078592,0.0001739422,0.0003868797,0.000005373971],"category_scores_gemma":[0.0005506841,0.0001851856,0.00004987056,0.0004160715,0.0001192916,0.00001440539,0.00004437825,0.0002481181,7.319527e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004311247,"about_ca_system_score_gemma":0.00007947715,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001199202,"about_ca_topic_score_gemma":0.00003071402,"domain_scores_codex":[0.9986812,0.00006899699,0.0004311933,0.0003522777,0.0002029022,0.0002633661],"domain_scores_gemma":[0.9992604,0.00003982709,0.0002169548,0.000326035,0.0001035503,0.00005321199],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002254013,0.0001059177,0.001658607,0.0001230124,0.000006579759,8.385227e-7,0.000085958,0.0001784595,0.9966441,0.00001071458,0.00004587914,0.0009145363],"study_design_scores_gemma":[0.00169118,0.0001643317,0.001563843,0.0001177975,0.00001220651,0.00001928063,0.0002380203,0.03571858,0.9597918,0.00002311243,0.0004417658,0.0002180877],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.997867,0.0003059746,0.0007649894,0.00007862927,0.00003682078,0.0004975278,0.00001277091,0.00003834035,0.0003978719],"genre_scores_gemma":[0.9988861,0.00002214737,0.0005251883,0.0000462969,0.00009901626,0.00004569997,0.0003266744,0.00001781254,0.00003107147],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0368523,"threshold_uncertainty_score":0.7551648,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008272402462947106,"score_gpt":0.2420747785011274,"score_spread":0.2338023760381803,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}