{"id":"W2330618139","doi":"10.1021/bi301021u","title":"Identification of Tyr241 as a Key Catalytic Base in the Family 4 Glycoside Hydrolase BglT from <i>Thermotoga maritima</i>","year":2012,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Thermotoga maritima; Glycoside hydrolase; Chemistry; Glycoside; Identification (biology); Key (lock); Base (topology); Stereochemistry; Catalysis; Hydrolase; Biochemistry; Computational biology; Biology; Botany; Hydrolysis; Enzyme; Escherichia coli; Mathematics; Ecology; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001091855,0.0003179359,0.0002226916,0.0001104166,0.0001282618,0.0003439921,0.0002553256,0.0003880434,0.0005939985],"category_scores_gemma":[0.0001927451,0.0001117254,0.0002258627,0.00013716,0.0002360744,0.000157912,0.000191628,0.0003308503,0.0003970335],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003197692,"about_ca_system_score_gemma":0.0001857684,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009669519,"about_ca_topic_score_gemma":0.0005484858,"domain_scores_codex":[0.9999378,0.000008960207,0.00000507496,0.00001873304,0.00001992219,0.000009528263],"domain_scores_gemma":[0.9998837,0.00001498185,0.0000475838,0.000009374758,0.00001576407,0.00002864741],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001312619,0.00001224702,0.0006754811,0.00003294238,0.00000528977,0.0001281478,0.0000110716,0.0001037823,0.9973217,0.00006557582,0.00002262521,0.001489997],"study_design_scores_gemma":[0.000006855724,0.00005854582,0.005730489,0.000003829456,0.000009642072,0.0003973101,0.00001724647,0.0007042077,0.9915767,0.00004262896,0.001447218,0.000005269988],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955486,0.0004318802,0.003064581,0.00006345047,0.0000149386,0.00001219794,0.0002677632,0.00005172685,0.0005448653],"genre_scores_gemma":[0.9936473,0.000227725,0.003765465,0.00002807643,0.000006088335,0.000012041,0.0008874022,0.00003249353,0.001393363],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009669519,"threshold_uncertainty_score":0.002320051,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007577748596936253,"score_gpt":0.2286092253739702,"score_spread":0.2210314767770339,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}