{"id":"W2334852980","doi":"10.1091/mbc.e14-07-1186","title":"Fast protein-depletion system utilizing tetracycline repressible promoter and N-end rule in yeast","year":2014,"lang":"en","type":"article","venue":"Molecular Biology of the Cell","topic":"Ubiquitin and proteasome pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Biology; Fusion protein; Tetracycline; Promoter; Protein degradation; Fusion gene; Cell biology; Green fluorescent protein; Gene; Molecular biology; Genetics; Gene expression; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000305358,0.0005170733,0.0004829731,0.0003396421,0.0002822054,0.0004495193,0.0006207242,0.0003541933,0.0008012685],"category_scores_gemma":[0.000124124,0.0002708329,0.0003432121,0.0002829058,0.0002759836,0.0003299127,0.0005472862,0.0007390593,0.0007939122],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004540908,"about_ca_system_score_gemma":0.0002918335,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000916498,"about_ca_topic_score_gemma":0.001329603,"domain_scores_codex":[0.9997297,0.00002800987,0.0000391825,0.00008422958,0.00008759218,0.00003120671],"domain_scores_gemma":[0.9998205,0.00003072217,0.00003851248,0.0000499592,0.00002704033,0.0000332139],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003526406,0.00001369105,0.00006809855,0.00003408626,0.000003632946,0.00003366153,0.00001190072,0.00006020512,0.9981672,0.0001224385,0.00004709767,0.001402791],"study_design_scores_gemma":[0.000009630161,0.00009357085,0.0006195001,0.000005256511,0.00001045259,0.0001532723,0.00001080353,0.001014959,0.9945779,0.00005543313,0.003438339,0.00001097655],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8027221,0.003032243,0.1852875,0.0002972365,0.0001828455,0.0003234038,0.002958241,0.002225399,0.00297103],"genre_scores_gemma":[0.8951138,0.002411954,0.08638997,0.000116982,0.00003059502,0.000343719,0.003948747,0.000473777,0.01117054],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000916498,"threshold_uncertainty_score":0.003294706,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007519998871526692,"score_gpt":0.225967196597069,"score_spread":0.2184471977255423,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}