{"id":"W2336525161","doi":"10.1371/journal.pone.0139137","title":"Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Streptavidin; Biotinylation; Cysteine; Covalent bond; Chemistry; Peptide; Protein tag; Recombinant DNA; Affinity chromatography; Combinatorial chemistry; Binding site; Biotin; Biophysics; Biochemistry; Fusion protein; Enzyme; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004551484,0.0005211097,0.0004194066,0.0002012364,0.0001854479,0.0004621948,0.0005833466,0.0005387161,0.0006149213],"category_scores_gemma":[0.0003868241,0.0003845134,0.0004146872,0.0003302503,0.0003079923,0.0002731872,0.0005514236,0.0007592037,0.0007418381],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000432252,"about_ca_system_score_gemma":0.0005104408,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000848335,"about_ca_topic_score_gemma":0.001269349,"domain_scores_codex":[0.9995634,0.00007054543,0.00004758589,0.00006318563,0.0001718657,0.00008352492],"domain_scores_gemma":[0.9997898,0.00003269727,0.00004413675,0.00002292795,0.00005531017,0.00005518572],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001661657,0.00001561757,0.00005828742,0.00002852826,0.000003861054,0.00003461682,0.00001523972,0.00008432099,0.9987321,0.0001526863,0.0000245752,0.000833488],"study_design_scores_gemma":[0.00001180455,0.00007727306,0.0002297084,0.000002570573,0.000005473257,0.0001110941,0.00001500831,0.001755124,0.9960731,0.00004812571,0.001665563,0.000005227736],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8838183,0.0009499574,0.1119251,0.0002537119,0.00008205676,0.0002574134,0.0003327065,0.0004200366,0.001960734],"genre_scores_gemma":[0.9060727,0.0006514931,0.08562495,0.0001139161,0.00001524693,0.0002305372,0.0007409031,0.0001285515,0.006421644],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.000848335,"threshold_uncertainty_score":0.003136218,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02743196339250287,"score_gpt":0.2123552205010596,"score_spread":0.1849232571085568,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}