{"id":"W2336525161","doi":"10.1371/journal.pone.0139137","title":"Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Streptavidin; Biotinylation; Cysteine; Covalent bond; Chemistry; Peptide; Protein tag; Recombinant DNA; Affinity chromatography; Combinatorial chemistry; Binding site; Biotin; Biophysics; Biochemistry; Fusion protein; Enzyme; Biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.000626089,0.0002733886,0.0004177648,0.0001764217,0.0001901615,0.00004720147,0.0001376589,0.0002478663,0.000003523972],"category_scores_gemma":[0.0004171079,0.0002512871,0.00004117195,0.0003011271,0.0001052638,0.00002273382,0.0002489647,0.0003086938,0.000004416234],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006526044,"about_ca_system_score_gemma":0.00008058419,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009537964,"about_ca_topic_score_gemma":0.00006462828,"domain_scores_codex":[0.9983929,0.00006084812,0.0003646929,0.0005684751,0.0002654283,0.0003476859],"domain_scores_gemma":[0.9989694,0.00004998302,0.0002213182,0.0003357515,0.0002008177,0.0002227455],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001766562,0.0003489848,0.02275218,0.0003645575,0.0003582925,0.00000422577,0.0006842578,0.0001441117,0.9749868,0.0000416056,0.0000212008,0.0001171484],"study_design_scores_gemma":[0.001395363,0.0009966543,0.002873115,0.001044811,0.0002137881,0.000008540193,0.001321152,0.0008210976,0.9907645,0.00002025671,0.00006833099,0.0004724178],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998332,0.0002679802,0.0001508394,0.0002509299,0.00004434095,0.0008169655,0.00004093007,0.0000427479,0.0000532974],"genre_scores_gemma":[0.9882075,0.0002649924,0.01116272,0.000009295633,0.00004943229,0.00005449617,0.00005951857,0.00003395381,0.0001581018],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01987907,"threshold_uncertainty_score":0.9999939,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02743196339250287,"score_gpt":0.2123552205010596,"score_spread":0.1849232571085568,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}