{"id":"W2341141507","doi":"10.1371/journal.pone.0127958","title":"A Novel and Simple Method for Rapid Generation of Recombinant Porcine Adenoviral Vectors for Transgene Expression","year":2015,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Chinese Academy of Agricultural Sciences; Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences; National Natural Science Foundation of China","keywords":"Recombinant DNA; Transduction (biophysics); Cloning (programming); Plasmid; Biology; Viral vector; Virology; Molecular biology; Electroporation; Vector (molecular biology); Adenoviridae; Gene delivery; Genetic enhancement; Transgene; Gene; Cloning vector; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003790639,0.0007592739,0.0004991172,0.0006996165,0.0004963195,0.0003873526,0.0004614169,0.0005238556,0.002121749],"category_scores_gemma":[0.000240458,0.0003977045,0.0007667497,0.0004612399,0.0002976745,0.0004760288,0.0004995491,0.001195772,0.001574252],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002044096,"about_ca_system_score_gemma":0.0005209712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005498852,"about_ca_topic_score_gemma":0.000861978,"domain_scores_codex":[0.999589,0.00004150586,0.00004591603,0.0001252415,0.0001506096,0.00004774894],"domain_scores_gemma":[0.9999019,0.00001591444,0.00001893809,0.00002271184,0.00002278752,0.00001776987],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002824432,0.000019979,0.0001791411,0.0001687024,0.000009595175,0.0001153276,0.00003213964,0.00008117178,0.9862883,0.0004147683,0.0005055035,0.0121571],"study_design_scores_gemma":[0.00003662048,0.0005116492,0.00282288,0.00005231962,0.00007566746,0.003385485,0.00003662349,0.002461168,0.9192879,0.0004530156,0.07080705,0.00006961113],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1681962,0.01031172,0.8021561,0.0005506403,0.001055667,0.001568213,0.003156784,0.002636284,0.01036843],"genre_scores_gemma":[0.3887317,0.01121475,0.5711592,0.0004989776,0.0002106457,0.001385145,0.01183895,0.0004251602,0.01453543],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002121749,"threshold_uncertainty_score":0.00709796,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1605729780247031,"score_gpt":0.3470772575479039,"score_spread":0.1865042795232008,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}