{"id":"W2344877955","doi":"10.1021/acs.bioconjchem.6b00124","title":"pHAST (pH-Driven Aptamer Switch for Thrombin) Catch-and-Release of Target Protein","year":2016,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Health Canada; Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada; Carleton University","keywords":"Aptamer; Chemistry; Förster resonance energy transfer; Thrombin; Biosensor; Biophysics; Protonation; G-quadruplex; Conformational change; Fluorescence; DNA; Combinatorial chemistry; Biochemistry; Ion; Molecular biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002346456,0.0002792687,0.0002747906,0.0001357966,0.00009358558,0.0002085299,0.0002424757,0.0003884675,0.001075337],"category_scores_gemma":[0.000282235,0.0002121033,0.0002289035,0.000121954,0.0002124042,0.0002566095,0.0002134082,0.0004698043,0.0004532609],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002596606,"about_ca_system_score_gemma":0.0001569384,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001462518,"about_ca_topic_score_gemma":0.0002833839,"domain_scores_codex":[0.9997619,0.00003307653,0.00001961855,0.00007520388,0.00008004427,0.0000301275],"domain_scores_gemma":[0.9998228,0.00005043424,0.00005260812,0.00001652791,0.00002293674,0.00003480363],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002183606,0.00000639514,0.00002870064,0.00001311137,0.000001476768,0.0000228701,0.000004698682,0.00005400685,0.9989041,0.00005275131,0.00001590107,0.0008742404],"study_design_scores_gemma":[0.000007949113,0.00007625078,0.0002262032,7.817157e-7,0.000001911809,0.00009767292,0.000001837999,0.0004056517,0.9986073,0.00001555018,0.0005559806,0.000002825238],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9143531,0.001185106,0.07987615,0.0001562808,0.0001158565,0.0001500397,0.00054261,0.0007246386,0.002896238],"genre_scores_gemma":[0.9338118,0.0005920158,0.05858048,0.0002423306,0.00002840635,0.0001391322,0.0004785223,0.00006699643,0.006060311],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001075337,"threshold_uncertainty_score":0.003597319,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009142568516936287,"score_gpt":0.2517476695865911,"score_spread":0.2426051010696548,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}