{"id":"W2344877955","doi":"10.1021/acs.bioconjchem.6b00124","title":"pHAST (pH-Driven Aptamer Switch for Thrombin) Catch-and-Release of Target Protein","year":2016,"lang":"en","type":"article","venue":"Bioconjugate Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"Health Canada; Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada; Carleton University","keywords":"Aptamer; Chemistry; Förster resonance energy transfer; Thrombin; Biosensor; Biophysics; Protonation; G-quadruplex; Conformational change; Fluorescence; DNA; Combinatorial chemistry; Biochemistry; Ion; Molecular biology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001143786,0.0002298069,0.0002633042,0.00001983136,0.00006109397,0.00001110804,0.0001689445,0.000232993,0.0000106622],"category_scores_gemma":[0.0001127142,0.0001630988,0.0001582278,0.00006183579,0.0002814989,0.000005764097,0.00009771622,0.0000504795,0.000001568249],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001658306,"about_ca_system_score_gemma":0.00005993835,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009300223,"about_ca_topic_score_gemma":0.000002240922,"domain_scores_codex":[0.9988258,0.00001550596,0.0002911563,0.0004899859,0.0001056281,0.0002718756],"domain_scores_gemma":[0.9991072,0.00001356041,0.0001936662,0.0004106176,0.0001660769,0.0001088769],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001187451,0.00007062636,0.000220089,0.00009422779,0.00008007974,9.979059e-7,0.000005847165,2.030631e-7,0.996136,0.00002380327,0.000695788,0.002553628],"study_design_scores_gemma":[0.0007690706,0.0001423995,0.0000308698,0.00007986772,0.00003677863,0.00001035377,0.00002767367,0.00001143393,0.9865153,0.0003259469,0.01178537,0.0002649199],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9932142,0.0002777246,0.005127472,0.0004405568,0.00002138204,0.0003698469,0.0002098653,0.00004394991,0.0002950339],"genre_scores_gemma":[0.9885301,0.0001436239,0.009092375,0.00006586801,0.0001709876,0.00006814493,0.0001042654,0.00003145586,0.001793161],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01108958,"threshold_uncertainty_score":0.6650975,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009142568516936287,"score_gpt":0.2517476695865911,"score_spread":0.2426051010696548,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}