{"id":"W2345658192","doi":"10.1016/j.mimet.2016.04.015","title":"Use of flow cytometry and PCR analysis to detect 5-carboxyfluoroscein-stained obligate intracellular bacteria Lawsonia intracellularis invasion of McCoy cells","year":2016,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"Veterinary medicine and infectious diseases","field":"Veterinary","cited_by":10,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"School for Public Health Research; Ministry of Agriculture - Saskatchewan; Natural Sciences and Engineering Research Council of Canada; Alberta Livestock and Meat Agency; Saskatchewan Health Research Foundation; Ontario Pork","keywords":"Lawsonia intracellularis; Obligate; Flow cytometry; Biology; Bacteria; Microbiology; Intracellular parasite; Molecular biology; Genetics; Ecology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004373234,0.0007291353,0.0004958197,0.000926094,0.0003634855,0.0004281235,0.000510551,0.000536314,0.00117868],"category_scores_gemma":[0.0004996879,0.0002805825,0.0004378888,0.0004939159,0.000444219,0.0004692949,0.0002438965,0.0006993972,0.0006169521],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002492924,"about_ca_system_score_gemma":0.0003769414,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001261746,"about_ca_topic_score_gemma":0.001669217,"domain_scores_codex":[0.9994319,0.00007503738,0.0000703547,0.0001638878,0.0001618984,0.00009681855],"domain_scores_gemma":[0.9992889,0.000331593,0.0001173994,0.00008076111,0.0001378683,0.00004341844],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002529788,0.00002773033,0.0002756253,0.00002438073,0.000001855511,0.00001700695,0.00003096346,0.00002443269,0.998764,0.00004786211,0.000006352474,0.000754569],"study_design_scores_gemma":[0.000005226466,0.0001920903,0.004289119,0.00000662097,0.00001776259,0.00009735858,0.00005485097,0.001466444,0.9933101,0.00007254691,0.0004801288,0.00000780077],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8868856,0.0008710505,0.1065481,0.0001455272,0.000102204,0.0003155071,0.0009317772,0.0005190092,0.003681167],"genre_scores_gemma":[0.8474826,0.001130627,0.143649,0.0001537037,0.00004581553,0.000849729,0.002072356,0.00007414706,0.004542132],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001261746,"threshold_uncertainty_score":0.003943086,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08466974363322848,"score_gpt":0.3555390177809151,"score_spread":0.2708692741476866,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}