{"id":"W2374465858","doi":"","title":"Co-expression plasmid construction of vascular endothelial growth factor and Fas ligand and detection of its expression in cultivated cells","year":2003,"lang":"en","type":"article","venue":"Zhonghua shiyan waike zazhi","topic":"Angiogenesis and VEGF in Cancer","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"CAE (Canada)","funders":"","keywords":"Fas ligand; Lipofectamine; Molecular biology; Transfection; Flow cytometry; Complementary DNA; Expression vector; Western blot; Biology; Apoptosis; Recombinant DNA; Gene; Programmed cell death; Vector (molecular biology); Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004771729,0.0004586278,0.0006065464,0.0004520945,0.0003269787,0.0005744102,0.0004165546,0.0005082919,0.001825815],"category_scores_gemma":[0.0004176602,0.0002686034,0.0003581182,0.000678175,0.0002824479,0.0004586269,0.0004059198,0.00094444,0.001214787],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004545336,"about_ca_system_score_gemma":0.0004326026,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004816758,"about_ca_topic_score_gemma":0.0004352122,"domain_scores_codex":[0.9990938,0.00009564571,0.00009895815,0.0002788158,0.0003388675,0.00009401274],"domain_scores_gemma":[0.9997354,0.00003810414,0.00005380108,0.00004414942,0.00009530665,0.00003319011],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001055335,0.00006677481,0.0005887282,0.0001512772,0.00001175094,0.0001096464,0.00008561653,0.0001714722,0.9915311,0.0009632912,0.0003367185,0.00587807],"study_design_scores_gemma":[0.00003814591,0.0002833746,0.001356972,0.00001835848,0.000045386,0.0004923531,0.00004741969,0.003710022,0.9735664,0.0001847187,0.02024001,0.00001688059],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6836401,0.005686887,0.2958395,0.0006798776,0.0006059406,0.0009126472,0.003607993,0.001314268,0.007712849],"genre_scores_gemma":[0.812526,0.003229321,0.1504619,0.0002633292,0.0001182672,0.001388264,0.009359704,0.0001701712,0.022483],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001825815,"threshold_uncertainty_score":0.006107926,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008024280997013621,"score_gpt":0.2267084780364722,"score_spread":0.2186841970394586,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}