{"id":"W2398757272","doi":"10.1007/978-1-4939-0944-5_25","title":"A Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling","year":2014,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canada Research Chairs; University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Cholesterol; Dynamin; Membrane; Synaptic vesicle; Vesicle; Organelle; Lipid raft; Cell biology; Chemistry; Biochemistry; Biology; Endocytosis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006538218,0.0009385975,0.0005950184,0.0006984833,0.000874721,0.0004362614,0.0007837434,0.001035786,0.002165959],"category_scores_gemma":[0.0006902441,0.0004496818,0.0005211234,0.0005817947,0.0004425601,0.0006930643,0.0006947923,0.002390204,0.001348241],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004650829,"about_ca_system_score_gemma":0.0009135482,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001019621,"about_ca_topic_score_gemma":0.002471418,"domain_scores_codex":[0.9995199,0.00007642418,0.00005521231,0.000111828,0.0001601464,0.00007646137],"domain_scores_gemma":[0.9995891,0.0001245523,0.00005387813,0.00008494873,0.00009716634,0.00005038804],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003466288,0.0000321055,0.00004526492,0.00006530475,0.000007764465,0.00004442071,0.00001606071,0.00002894315,0.9957065,0.0002110495,0.0002227484,0.003585257],"study_design_scores_gemma":[0.00003458878,0.0001096635,0.0007115011,0.00001567429,0.00003107857,0.0003292855,0.000018352,0.0006206281,0.9879671,0.000258027,0.00987656,0.00002747669],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2121498,0.00810632,0.7621105,0.002095126,0.001051275,0.002361059,0.002285758,0.001325106,0.008515004],"genre_scores_gemma":[0.370714,0.01007557,0.5786696,0.002181098,0.0003087817,0.003862741,0.006212923,0.0005292084,0.02744599],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002165959,"threshold_uncertainty_score":0.007245898,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01630860891899064,"score_gpt":0.388287370316744,"score_spread":0.3719787613977534,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}