{"id":"W2399590689","doi":"10.1007/978-1-4939-0799-1_19","title":"Detection of Protein Posttranslational Modifications from Whole-Cell Extracts in Saccharomyces cerevisiae","year":2014,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"DNA Repair Mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary; University of Saskatchewan","funders":"Canadian Institutes of Health Research","keywords":"SUMO protein; Saccharomyces cerevisiae; Ubiquitin; Cell biology; Yeast; Biology; Phosphorylation; Function (biology); Biochemistry; Model organism; Cell; SUMO enzymes; F-box protein; Computational biology; Chemistry; Ubiquitin ligase; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001552659,0.0004328281,0.0002332958,0.0003495456,0.0001674529,0.0004211445,0.0002606738,0.0002298319,0.0006711342],"category_scores_gemma":[0.000209284,0.0002141108,0.0002779573,0.0003094477,0.0001738134,0.0002908217,0.0001988436,0.0005571919,0.0004510169],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002581117,"about_ca_system_score_gemma":0.0001869921,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00171168,"about_ca_topic_score_gemma":0.002339408,"domain_scores_codex":[0.9999408,0.000005981562,0.000009975569,0.00001997582,0.00001438962,0.000008888986],"domain_scores_gemma":[0.9998369,0.00004797278,0.00002137156,0.00003630217,0.00002409326,0.00003341224],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009942816,0.00001129686,0.0002879569,0.00003286227,0.000006456972,0.00002266002,0.00001459736,0.00003365461,0.9984685,0.00005113259,0.00002832319,0.0009431461],"study_design_scores_gemma":[0.00001128017,0.00008442017,0.01177238,0.000006058824,0.00002438202,0.0001524104,0.000048851,0.0005808799,0.9854145,0.0001009988,0.001796478,0.00000740149],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9853976,0.002341981,0.008324649,0.0001615045,0.00005800061,0.00003058598,0.002495361,0.0001807443,0.001009579],"genre_scores_gemma":[0.9694407,0.003240375,0.01423283,0.0001357972,0.00003263506,0.00004618769,0.008001631,0.0001280966,0.004741825],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00171168,"threshold_uncertainty_score":0.003403425,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01877807261019846,"score_gpt":0.3308042501378653,"score_spread":0.3120261775276668,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}