{"id":"W2401473574","doi":"10.1385/1-59259-182-5:155","title":"Measuring DNA Demethylase Activity In Vitro","year":2003,"lang":"en","type":"article","venue":"Humana Press eBooks","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Cancer Institute","keywords":"Demethylation; DNA demethylation; CpG site; HpaII; DNA; DNA methylation; Methylation; Cytosine; Bisulfite; Restriction enzyme; Biochemistry; Methylated DNA immunoprecipitation; Chemistry; Bisulfite sequencing; 5-Methylcytosine; Enzyme; Molecular biology; Methyltransferase; Biology; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002985823,0.0001252655,0.000114048,0.00004417992,0.00006106868,0.00002396355,0.000103079,0.0001129199,0.000004539201],"category_scores_gemma":[0.00007843802,0.0001335501,0.00005605613,0.00001325781,0.00003567012,0.000002300934,0.00004764629,0.00009270743,0.000001975125],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001442379,"about_ca_system_score_gemma":0.00002909015,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007366826,"about_ca_topic_score_gemma":0.00009802659,"domain_scores_codex":[0.9990788,0.0001514646,0.000143424,0.0002878926,0.0001151028,0.0002233157],"domain_scores_gemma":[0.9995309,0.00001162746,0.00005645895,0.000305629,0.00003482394,0.00006052036],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004296018,0.0000317201,0.00006931346,0.00001312184,0.0000142625,0.000003252327,0.00004666573,0.00005719564,0.9792087,0.0003943557,0.00001325676,0.02010517],"study_design_scores_gemma":[0.0003943612,0.00003759634,0.0007014062,0.000005000738,0.000007570737,6.166459e-7,0.000005537201,0.00003053721,0.9547886,0.0002813448,0.04359688,0.0001506106],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9475552,0.003304217,0.001286524,0.000003103132,0.00009488638,0.0001626589,0.000003712225,0.00001090899,0.04757873],"genre_scores_gemma":[0.9983005,0.0001358034,0.0004536116,0.00002470828,0.0000667265,0.00003084214,0.000007799528,0.00002037467,0.0009596205],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05074525,"threshold_uncertainty_score":0.5446016,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06951485157431182,"score_gpt":0.2728931930159303,"score_spread":0.2033783414416185,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}