{"id":"W2401473574","doi":"10.1385/1-59259-182-5:155","title":"Measuring DNA Demethylase Activity In Vitro","year":2003,"lang":"en","type":"article","venue":"Humana Press eBooks","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Cancer Institute","keywords":"Demethylation; DNA demethylation; CpG site; HpaII; DNA; DNA methylation; Methylation; Cytosine; Bisulfite; Restriction enzyme; Biochemistry; Methylated DNA immunoprecipitation; Chemistry; Bisulfite sequencing; 5-Methylcytosine; Enzyme; Molecular biology; Methyltransferase; Biology; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00155487,0.002300149,0.001472238,0.001608433,0.0005732144,0.001269995,0.001133675,0.001110197,0.003156035],"category_scores_gemma":[0.001920013,0.0009815735,0.0008929805,0.00164789,0.0004823465,0.0007187171,0.0009752445,0.002951609,0.003985886],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006962688,"about_ca_system_score_gemma":0.0004994915,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009896113,"about_ca_topic_score_gemma":0.002546358,"domain_scores_codex":[0.996821,0.0008446369,0.0003436137,0.0008475337,0.0008171814,0.0003259789],"domain_scores_gemma":[0.9980361,0.0008148447,0.0002014589,0.0003396738,0.0003910432,0.0002168575],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006949426,0.0001435652,0.000254442,0.000179626,0.00003139661,0.00001797397,0.00005408885,0.0002419641,0.9941788,0.0001835554,0.0002263514,0.004418879],"study_design_scores_gemma":[0.00001501586,0.0003432657,0.0008045784,0.00002243174,0.00004497711,0.00005325015,0.00001635892,0.0008939431,0.9923359,0.0001656631,0.005288749,0.00001591864],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4224615,0.03426851,0.4852378,0.0007949098,0.001502263,0.00221952,0.01786382,0.004811793,0.03083991],"genre_scores_gemma":[0.5180477,0.02671298,0.3604198,0.0006570321,0.000537492,0.001873208,0.04338168,0.0007999219,0.04757013],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003156035,"threshold_uncertainty_score":0.01055801,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06951485157431182,"score_gpt":0.2728931930159303,"score_spread":0.2033783414416185,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}