{"id":"W2404001249","doi":"10.1016/bs.mie.2014.12.013","title":"Expression of G Protein-Coupled Receptors in Mammalian Cells","year":2015,"lang":"en","type":"article","venue":"Methods in enzymology on CD-ROM/Methods in enzymology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba; Children's Hospital Research Institute of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; Manitoba Medical Service Foundation; Research Manitoba; Manitoba Health Research Council","keywords":"G protein-coupled receptor; Biology; Receptor; Transfection; Heterologous expression; Cell biology; Computational biology; Cell; Extracellular; Heterologous; Intracellular; Membrane protein; Gene; Biochemistry; Membrane; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007465823,0.0004986131,0.0006640181,0.0004631571,0.0004239789,0.001040758,0.0009882894,0.0006451344,0.001959285],"category_scores_gemma":[0.00044502,0.0005562079,0.000736284,0.000840637,0.0005803508,0.0005456941,0.0004865039,0.001741707,0.003069136],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000589907,"about_ca_system_score_gemma":0.0004392737,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005834408,"about_ca_topic_score_gemma":0.0009166966,"domain_scores_codex":[0.9988674,0.0002145855,0.0001652265,0.0001501899,0.0004440686,0.0001586049],"domain_scores_gemma":[0.9997453,0.00007665933,0.00002795224,0.00008334778,0.00003553093,0.00003111091],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000635491,0.00002344034,0.00004489054,0.00007424054,0.000007570405,0.00002587574,0.00003062837,0.0001413635,0.995216,0.001442376,0.0002828878,0.00264714],"study_design_scores_gemma":[0.00001647364,0.00005638877,0.0002300389,0.000007498203,0.00001383544,0.0000544732,0.000009570594,0.0009893613,0.9856961,0.0001897033,0.01273168,0.000004872626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.4681932,0.00758871,0.4770441,0.00158463,0.0009250196,0.001228373,0.01563257,0.003877338,0.02392595],"genre_scores_gemma":[0.5522436,0.008878855,0.3746181,0.000605303,0.0001730271,0.001207968,0.02452254,0.001249711,0.03650081],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001959285,"threshold_uncertainty_score":0.006554484,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04493733419771653,"score_gpt":0.3763365467262813,"score_spread":0.3313992125285648,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}