{"id":"W2404859095","doi":"10.1007/978-1-4939-3664-9_8","title":"Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis","year":2016,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Connexins and lens biology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Ottawa","funders":"Canadian Institutes of Health Research","keywords":"Interactome; Immunoprecipitation; Membrane protein; Integral membrane protein; Cell fractionation; Gap junction; Cell biology; Chemistry; Crosstalk; Biology; Peripheral membrane protein; Membrane; Computational biology; Intracellular; Biochemistry; Physics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000983425,0.00124134,0.001432304,0.001210942,0.001899698,0.001111601,0.00136583,0.0009282809,0.003572673],"category_scores_gemma":[0.001216182,0.0008613589,0.00141797,0.001575867,0.0005745685,0.00102468,0.001484185,0.004128114,0.003565084],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007588522,"about_ca_system_score_gemma":0.0007267877,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001494043,"about_ca_topic_score_gemma":0.004196407,"domain_scores_codex":[0.9989327,0.0001168583,0.0001473634,0.0003059302,0.0002577995,0.0002394144],"domain_scores_gemma":[0.9993031,0.0001916704,0.0000669392,0.0002163925,0.0001385168,0.00008329875],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001708201,0.0001244506,0.0003666837,0.000137083,0.00005655882,0.0001089755,0.00006556297,0.0002468763,0.9952034,0.0008815217,0.0004957723,0.002142354],"study_design_scores_gemma":[0.00007490984,0.0001342576,0.006992565,0.00003959242,0.0001199538,0.0002322231,0.00009898346,0.004951309,0.9696571,0.001063707,0.01660291,0.00003250261],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5309377,0.005383501,0.4194102,0.002132381,0.001175852,0.004175991,0.02238959,0.002203387,0.01219139],"genre_scores_gemma":[0.557556,0.00553678,0.347118,0.001480019,0.0003434555,0.007149631,0.06412009,0.001406284,0.01528983],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.003572673,"threshold_uncertainty_score":0.0119518,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01652223127373312,"score_gpt":0.3967050843084582,"score_spread":0.380182853034725,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}