{"id":"W2407589310","doi":"10.1007/978-1-61779-160-4_22","title":"Tandem Affinity Purification and Identification of Heterotrimeric G Protein-Associated Proteins","year":2011,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Heterotrimeric G protein; G protein-coupled receptor; G protein; GTPase-activating protein; Cell biology; G beta-gamma complex; Effector; Biology; Gq alpha subunit; Chemistry; Signal transduction; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004197237,0.0007156965,0.0006736841,0.0008071617,0.0005322873,0.0005409067,0.0008484995,0.0006364326,0.001167826],"category_scores_gemma":[0.000373982,0.0002698911,0.0005439008,0.001076666,0.0002274863,0.0003032034,0.0006607767,0.001116968,0.00239748],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005179691,"about_ca_system_score_gemma":0.0003174455,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007923832,"about_ca_topic_score_gemma":0.001612844,"domain_scores_codex":[0.9994563,0.00006988676,0.00004441908,0.0001278851,0.0002023336,0.00009912528],"domain_scores_gemma":[0.9997867,0.00003141851,0.00003066426,0.00003516871,0.00007423833,0.00004185591],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003060344,0.00003082282,0.00009832921,0.00004970869,0.00001107702,0.00005052463,0.000019401,0.00006082432,0.9969079,0.0001809923,0.0002214569,0.002338322],"study_design_scores_gemma":[0.00003209771,0.0001803855,0.004448021,0.00002143013,0.0000452161,0.0008337351,0.00007450837,0.003285368,0.9657231,0.0005213579,0.02481082,0.00002391417],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7644457,0.01831977,0.1979238,0.00207946,0.0004308372,0.0008743712,0.006978385,0.001059726,0.007887831],"genre_scores_gemma":[0.7432616,0.01863565,0.1837173,0.001488391,0.0002458199,0.001111217,0.02910546,0.0004196258,0.02201495],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001167826,"threshold_uncertainty_score":0.003906786,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03216474117281629,"score_gpt":0.3374844910139299,"score_spread":0.3053197498411136,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}