{"id":"W2410193487","doi":"10.1007/978-1-59745-188-8_18","title":"Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line","year":2008,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Bacillus and Francisella bacterial research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute for Biological Sciences","funders":"","keywords":"Chromatography; Mass spectrometry; Peptide; Chemistry; Electrospray ionization; Francisella tularensis; Tandem mass spectrometry; Protein mass spectrometry; Electrospray; Sample preparation; Bottom-up proteomics; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002779557,0.0005749842,0.0004886239,0.0004653785,0.0002675211,0.00046907,0.0002443162,0.000529254,0.0004199314],"category_scores_gemma":[0.0002199989,0.0001458531,0.0002065398,0.0002467308,0.0002199345,0.0003569946,0.0002303439,0.0003932602,0.0004972327],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001935558,"about_ca_system_score_gemma":0.0001950126,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003382458,"about_ca_topic_score_gemma":0.001112944,"domain_scores_codex":[0.9998728,0.00001852767,0.00001275567,0.0000392172,0.00004288726,0.00001380049],"domain_scores_gemma":[0.9999024,0.00005107044,0.0000125054,0.00000901378,0.00001783429,0.000007156962],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002168115,0.00001074528,0.0001623149,0.00003024734,0.000003831497,0.00002292722,0.00000963785,0.00002046372,0.9980838,0.00001665732,0.00001932362,0.001598329],"study_design_scores_gemma":[0.00001216021,0.0001665408,0.003789102,0.000008341293,0.00002857292,0.000397288,0.00003312036,0.001354205,0.9919623,0.00008629616,0.002146216,0.00001580244],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8817927,0.00639807,0.1046802,0.00033733,0.0001294617,0.0003207121,0.002756292,0.0009923081,0.002592808],"genre_scores_gemma":[0.6290498,0.007731473,0.3522832,0.00100332,0.00009074017,0.0008005849,0.004794562,0.0002240379,0.004022347],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.0005749842,"threshold_uncertainty_score":0.00146997,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05009453203702218,"score_gpt":0.418740808215631,"score_spread":0.3686462761786088,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}