{"id":"W2410197854","doi":"10.1007/978-1-4939-1053-3_6","title":"Site-Saturation Mutagenesis by Overlap Extension PCR","year":2014,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Genomics and Phylogenetic Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":false,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; University of British Columbia","funders":"","keywords":"Saturated mutagenesis; Mutagenesis; Genetics; Computational biology; Biology; Overlap extension polymerase chain reaction; Gene; Stop codon; Directed evolution; Amino acid; Mutation; Mutant; Polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003554502,0.004669739,0.004112655,0.002190352,0.001782271,0.001372954,0.004132498,0.002620522,0.01165118],"category_scores_gemma":[0.001775805,0.004286687,0.003891559,0.001720525,0.002257837,0.0008766038,0.002176661,0.00747449,0.01363872],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001048183,"about_ca_system_score_gemma":0.002160881,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002238229,"about_ca_topic_score_gemma":0.005333248,"domain_scores_codex":[0.9933835,0.001498714,0.0006839975,0.001806296,0.001211564,0.001415965],"domain_scores_gemma":[0.9967102,0.001234282,0.0002578026,0.000847277,0.0005159806,0.0004343427],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003576163,0.0003047105,0.0002029661,0.0003855465,0.00007419649,0.0001709053,0.0001693248,0.0003142784,0.9905494,0.00106921,0.001133468,0.005268333],"study_design_scores_gemma":[0.0001874526,0.0002497718,0.0009916591,0.00004633926,0.0002491352,0.0003806602,0.00005613557,0.002105578,0.9758201,0.0003735739,0.01945574,0.00008395303],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1557075,0.001350465,0.8070914,0.0009265267,0.0008891912,0.005613043,0.00848033,0.01001366,0.009928064],"genre_scores_gemma":[0.2989281,0.002501289,0.6168483,0.001968869,0.000183801,0.004021036,0.03384037,0.006589331,0.03511893],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01165118,"threshold_uncertainty_score":0.03897709,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01314801671374131,"score_gpt":0.3316852110568839,"score_spread":0.3185371943431426,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}