{"id":"W2412092877","doi":"10.1007/7651_2016_322","title":"An Effective and Reliable Xeno-free Cryopreservation Protocol for Single Human Pluripotent Stem Cells","year":2016,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Canadian Institutes of Health Research","keywords":"Cryopreservation; Induced pluripotent stem cell; Dimethyl sulfoxide; Cell biology; Biology; Chemistry; Embryonic stem cell; Embryo; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005949002,0.0007707832,0.0005966207,0.0006145311,0.0008688134,0.0004611555,0.0009386659,0.0005184831,0.001566441],"category_scores_gemma":[0.0004899879,0.0003458589,0.0004661417,0.0005476502,0.0003200522,0.0008342941,0.0008991482,0.001128936,0.0009837141],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003793735,"about_ca_system_score_gemma":0.001033371,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006495097,"about_ca_topic_score_gemma":0.001564527,"domain_scores_codex":[0.9993066,0.00005964855,0.0001160955,0.0001201941,0.0003514614,0.00004607656],"domain_scores_gemma":[0.9997603,0.00003480066,0.00004035926,0.00005673317,0.00008744196,0.00002024943],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003044807,0.00003091397,0.0002072355,0.0001943247,0.00001361316,0.0001306963,0.00006399832,0.0003076595,0.9773585,0.000765743,0.0009812028,0.01991563],"study_design_scores_gemma":[0.00002067138,0.0001092061,0.002067253,0.00003814662,0.000103247,0.000796718,0.00003837321,0.001639793,0.9659916,0.0004926962,0.02865297,0.00004924902],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3213125,0.02420581,0.6256468,0.001359683,0.002015525,0.002217972,0.003369271,0.002065971,0.01780632],"genre_scores_gemma":[0.5445899,0.02116469,0.3945367,0.000755955,0.0005382428,0.003154064,0.009533525,0.0008840189,0.02484286],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001566441,"threshold_uncertainty_score":0.005240262,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03686391991409297,"score_gpt":0.4099166800831062,"score_spread":0.3730527601690132,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}