{"id":"W2412253570","doi":"10.2144/00282bm04","title":"Dominant Positive and Negative Selection Using Luciferase, Green Fluorescent Protein and β-Galactosidase Reporter Gene Fusions","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"Robarts Research Institute; Medical Research Council; Cancer Research Society; Medical Research Council Canada; ALS Society of Canada","keywords":"Reporter gene; Gene expression; Biology; Gene; Luciferase; Transgene; Nucleic acid; Molecular biology; Computational biology; Genetics; Biotechnology; Transfection","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001740364,0.001756439,0.00168303,0.001371193,0.0008332526,0.001177702,0.001731929,0.000784851,0.004351582],"category_scores_gemma":[0.000806238,0.0009971951,0.001206978,0.001359294,0.0008885416,0.0006045903,0.0009093432,0.00239033,0.004000772],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008524261,"about_ca_system_score_gemma":0.001264327,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00193066,"about_ca_topic_score_gemma":0.004437734,"domain_scores_codex":[0.9979405,0.0002486944,0.0002377902,0.0004315699,0.0007136363,0.0004278008],"domain_scores_gemma":[0.9989426,0.0002667324,0.0001165046,0.0001743381,0.0002167555,0.0002832196],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001312873,0.00003936726,0.00006528341,0.00005086653,0.000006447088,0.00004265784,0.00003949757,0.00001885563,0.9980863,0.000230398,0.0002109117,0.001078179],"study_design_scores_gemma":[0.00007616971,0.0001616462,0.001004971,0.00001589491,0.00006640973,0.0005309083,0.00004667484,0.0007291641,0.9839034,0.0001403426,0.01328861,0.00003582977],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.5979335,0.006553494,0.3528075,0.001820454,0.002036958,0.002978986,0.01148202,0.005647973,0.01873909],"genre_scores_gemma":[0.5562918,0.01078549,0.2832298,0.001720941,0.0002898845,0.00341013,0.04123921,0.004049556,0.09898327],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.004351582,"threshold_uncertainty_score":0.01455748,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01108753294486415,"score_gpt":0.2710171694169883,"score_spread":0.2599296364721242,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}