{"id":"W2412253570","doi":"10.2144/00282bm04","title":"Dominant Positive and Negative Selection Using Luciferase, Green Fluorescent Protein and β-Galactosidase Reporter Gene Fusions","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"Robarts Research Institute; Medical Research Council; Cancer Research Society; Medical Research Council Canada; ALS Society of Canada","keywords":"Reporter gene; Gene expression; Biology; Gene; Luciferase; Transgene; Nucleic acid; Molecular biology; Computational biology; Genetics; Biotechnology; Transfection","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001686218,0.0001827575,0.0001494178,0.00005512027,0.0001709383,0.00004042232,0.00007289569,0.0002650538,0.00003015269],"category_scores_gemma":[0.00003231881,0.0001575704,0.00004082908,0.0001232048,0.000215561,0.0000160814,0.00008953668,0.0001255898,9.156147e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002556348,"about_ca_system_score_gemma":0.00006163619,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006300441,"about_ca_topic_score_gemma":0.00005187035,"domain_scores_codex":[0.9988872,0.00006205632,0.0001958541,0.0004541817,0.000130083,0.0002705611],"domain_scores_gemma":[0.9995191,0.000005975737,0.00006009712,0.0001843085,0.0001076565,0.0001228352],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001518863,0.00005114952,0.001426061,0.00002178223,0.00001681662,0.00001130211,0.00004116353,4.453079e-8,0.9834025,0.000004583266,0.0001249161,0.01474783],"study_design_scores_gemma":[0.0002018609,0.0003012197,0.00259802,0.00008655721,0.00001475579,0.0001611955,0.00002707289,0.000131979,0.9953309,0.00003753293,0.0008971698,0.0002117146],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976527,0.0003435635,0.001017924,0.0002605409,0.000007176076,0.0005651348,0.00002138837,0.00002788622,0.0001036738],"genre_scores_gemma":[0.9931609,0.0009256253,0.005128107,0.00008765834,0.0001415666,0.00004977469,0.00004307615,0.00001995546,0.0004433467],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01453611,"threshold_uncertainty_score":0.6425533,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01108753294486415,"score_gpt":0.2710171694169883,"score_spread":0.2599296364721242,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}