{"id":"W2412324688","doi":"10.1107/s1744309113018927","title":"Cloning, expression, purification and preliminary X-ray diffraction studies of a novel AB<sub>5</sub>toxin","year":2013,"lang":"en","type":"article","venue":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity","funders":"National Health and Medical Research Council; Australian Research Council; Medical Research Council; Australian Synchrotron; Pfizer","keywords":"Escherichia coli; Protein subunit; Citrobacter freundii; Toxin; Biology; Virulence factor; Cloning (programming); Serine protease; Microbiology; Molecular biology; Chemistry; Virulence; Protease; Biochemistry; Enzyme; Enterobacteriaceae; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002668206,0.0003549811,0.0003955672,0.0002488754,0.0003358585,0.0006411066,0.0004911555,0.0004024394,0.0006055748],"category_scores_gemma":[0.0003253852,0.0002129594,0.0003827038,0.0009911016,0.000279602,0.0003315029,0.0002790052,0.0009000066,0.0006756964],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009350699,"about_ca_system_score_gemma":0.0005166016,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.006214595,"about_ca_topic_score_gemma":0.003365374,"domain_scores_codex":[0.9997508,0.00003251679,0.00004066475,0.00004373772,0.00007820968,0.00005412851],"domain_scores_gemma":[0.9998511,0.00002533087,0.00002637998,0.00001602077,0.00004307813,0.00003818007],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001804987,0.0001239924,0.001373184,0.0001355729,0.00001912836,0.0005862124,0.0001772698,0.0007894363,0.9917775,0.0005968857,0.0004288777,0.003811446],"study_design_scores_gemma":[0.00004710018,0.0001959326,0.02610334,0.00003544356,0.00006385594,0.002155686,0.0002487293,0.003637413,0.9323419,0.0002909557,0.03484732,0.00003234713],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9853536,0.003273866,0.006333833,0.0006570304,0.00006497279,0.0000672234,0.002323234,0.00005534959,0.001870917],"genre_scores_gemma":[0.9256196,0.008259533,0.0326637,0.0003652915,0.0000501609,0.00007439831,0.02223271,0.00009354235,0.01064107],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006214595,"threshold_uncertainty_score":0.01235682,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01612530065300501,"score_gpt":0.2654732795221569,"score_spread":0.2493479788691519,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}