{"id":"W2413110664","doi":"10.1007/978-1-4939-2871-2_14","title":"Expression and Purification of the Individual Bam Components BamB–E","year":2015,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Simon Fraser University","funders":"","keywords":"Bacterial outer membrane; Bama; Biology; Escherichia coli; Biochemistry; Bacteria; Membrane; Membrane protein; Affinity chromatography; Chemistry; Biophysics; Enzyme; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005130463,0.0006942889,0.0005512914,0.0005735054,0.0004220641,0.0008687584,0.0006793474,0.0005808461,0.002450289],"category_scores_gemma":[0.000822912,0.0006474094,0.0004613098,0.00127274,0.0004113507,0.0006273288,0.0005790281,0.002223533,0.003241624],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004793231,"about_ca_system_score_gemma":0.0005165297,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009862009,"about_ca_topic_score_gemma":0.0009952162,"domain_scores_codex":[0.9996563,0.00003164471,0.00003831436,0.00007387413,0.0001122481,0.00008754341],"domain_scores_gemma":[0.9995947,0.0000796909,0.00005724526,0.0000877136,0.00008223428,0.00009849912],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001969514,0.00006849194,0.0003800853,0.0001034064,0.00001088912,0.00005603247,0.00006003443,0.0001323898,0.9920403,0.0006877447,0.0006544837,0.005609174],"study_design_scores_gemma":[0.00001795102,0.00004472371,0.002259337,0.00001273999,0.00001416757,0.0001256404,0.0000282832,0.0004824301,0.979381,0.000219609,0.01740577,0.000008390452],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7942572,0.004090267,0.1769983,0.001368219,0.0004217952,0.0006515302,0.01390971,0.001379556,0.006923419],"genre_scores_gemma":[0.6494644,0.004719317,0.251765,0.0005917854,0.0001090689,0.0008498839,0.04637322,0.0008985882,0.04522869],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002450289,"threshold_uncertainty_score":0.00819701,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06202674601614713,"score_gpt":0.3699015602412531,"score_spread":0.307874814225106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}