{"id":"W2413143420","doi":"10.1385/1-59259-434-4:227","title":"Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique","year":2004,"lang":"en","type":"article","venue":"Humana Press eBooks","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; D-Wave Systems (Canada)","funders":"","keywords":"Transgenesis; Insertional mutagenesis; Human artificial chromosome; Biology; Genome; Genetics; Gene; Transgene; DNA; Mutagenesis; Microinjection; Computational biology; Chromosome; Mutation; Cell biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008102506,0.00009208368,0.0001195001,0.00003600851,0.00003740318,0.000006877615,0.00006230493,0.0001021592,0.000001056705],"category_scores_gemma":[0.000008096729,0.0000970105,0.00006954059,0.000008802307,0.00004996559,0.000001820416,0.0000174476,0.0000358138,6.441292e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000005939177,"about_ca_system_score_gemma":0.00002839551,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005746043,"about_ca_topic_score_gemma":0.00005390269,"domain_scores_codex":[0.9994556,0.00001121683,0.0002018108,0.0001774821,0.0000501901,0.0001036482],"domain_scores_gemma":[0.999725,0.000002992223,0.00006018845,0.0001412701,0.00004959746,0.0000208944],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005093484,0.00002273679,9.049195e-7,0.0001328067,0.00003268812,7.634711e-8,0.0002328003,0.0006009884,0.9942916,0.0007217961,0.00003410319,0.003878577],"study_design_scores_gemma":[0.0003594328,0.000343731,0.00003348685,0.00001883594,0.00003387524,0.000004690464,0.00001838753,0.00006459281,0.9970564,0.0001777099,0.001792082,0.0000967748],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9165962,0.001417246,0.0808256,0.000006467198,0.00008614638,0.0007257585,0.00002083086,0.00001445737,0.0003072725],"genre_scores_gemma":[0.9964544,0.00007950569,0.003037414,0.000006384681,0.0001766273,0.00008387549,0.00002641392,0.00001904619,0.0001163883],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07985812,"threshold_uncertainty_score":0.3955973,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03831782820614685,"score_gpt":0.3077123918545071,"score_spread":0.2693945636483602,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}