{"id":"W2413143420","doi":"10.1385/1-59259-434-4:227","title":"Pronuclear Microinjection of Purified Artificial Chromosomes for Generation of Transgenic Mice: Pick-and-Inject Technique","year":2004,"lang":"en","type":"article","venue":"Humana Press eBooks","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary; D-Wave Systems (Canada)","funders":"","keywords":"Transgenesis; Insertional mutagenesis; Human artificial chromosome; Biology; Genome; Genetics; Gene; Transgene; DNA; Mutagenesis; Microinjection; Computational biology; Chromosome; Mutation; Cell biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005525966,0.0006754024,0.0005674849,0.0006893803,0.0003933397,0.0004315776,0.0005301166,0.000556219,0.003229883],"category_scores_gemma":[0.0002949917,0.0003155515,0.0002783577,0.0004333894,0.0003559941,0.0002817535,0.0006280105,0.001417488,0.002578191],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003156135,"about_ca_system_score_gemma":0.0006717303,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000853016,"about_ca_topic_score_gemma":0.001925628,"domain_scores_codex":[0.9996819,0.00003347193,0.0000268665,0.00009036076,0.0001223122,0.00004517475],"domain_scores_gemma":[0.9998123,0.0000520772,0.00004322505,0.00003234481,0.00002093299,0.00003909038],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005741177,0.00002211014,0.0001331003,0.0001325927,0.00001384105,0.0001120367,0.00005454118,0.0001003559,0.9882541,0.001680186,0.0005513199,0.008888515],"study_design_scores_gemma":[0.00004553348,0.0001429197,0.001267424,0.00002556336,0.00004132698,0.0008139143,0.00001828695,0.00125493,0.9481102,0.000448905,0.04781147,0.00001943816],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1618318,0.007496244,0.8045373,0.0007477233,0.000718599,0.001506922,0.003430908,0.004977419,0.014753],"genre_scores_gemma":[0.3595197,0.01153241,0.576181,0.0004352549,0.0001145726,0.001693121,0.004901296,0.0006012795,0.04502143],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003229883,"threshold_uncertainty_score":0.01080507,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03831782820614685,"score_gpt":0.3077123918545071,"score_spread":0.2693945636483602,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}