{"id":"W2413770936","doi":"10.1534/g3.116.028662","title":"<i>N</i>-Ethyl-<i>N</i>-Nitrosourea (ENU) Mutagenesis Reveals an Intronic Residue Critical for<i>Caenorhabditis elegans</i>3′ Splice Site Function<i>in Vivo</i>","year":2016,"lang":"en","type":"article","venue":"G3 Genes Genomes Genetics","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"University of Michigan; National Institute of General Medical Sciences; American Heart Association; National Institute on Aging; National Institutes of Health; American Cancer Society","keywords":"Caenorhabditis elegans; Intron; Biology; RNA splicing; Genetics; Histone octamer; Splice site mutation; Transversion; Alternative splicing; Conserved sequence; Gene; Sequence motif; Molecular biology; Exon; RNA; Histone; Peptide sequence; Nucleosome","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001233097,0.0002971327,0.0001501227,0.0001132425,0.0001649544,0.0001595733,0.0002330443,0.0001942596,0.0008026108],"category_scores_gemma":[0.0001234658,0.0001412126,0.0002293851,0.0000802591,0.0002163546,0.00006377864,0.0001298597,0.0003908603,0.0002469379],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002870404,"about_ca_system_score_gemma":0.0003094088,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002290431,"about_ca_topic_score_gemma":0.005919489,"domain_scores_codex":[0.9998851,0.00001911953,0.00001212269,0.00002748978,0.0000359063,0.00002027774],"domain_scores_gemma":[0.999863,0.00002832583,0.00005207234,0.0000157419,0.00001100185,0.00002983688],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004620363,0.00001859165,0.0001814908,0.00001078466,0.000003456173,0.00004981744,0.000007534367,0.00007481837,0.9990689,0.0000624862,0.00001356508,0.0004623499],"study_design_scores_gemma":[0.00001169438,0.0001774748,0.004976547,0.00000757549,0.00001522531,0.0002508096,0.00001634849,0.0008246052,0.9926985,0.00003236958,0.0009832331,0.000005578831],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9954619,0.0001144351,0.003010108,0.00003338292,0.00001713791,0.00002919727,0.0001625748,0.00005994297,0.001111382],"genre_scores_gemma":[0.9943954,0.000137147,0.00395884,0.00004373239,0.00000338655,0.00001559958,0.0002816173,0.00003028572,0.001133954],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002290431,"threshold_uncertainty_score":0.004554212,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01182954901005023,"score_gpt":0.2748090894442939,"score_spread":0.2629795404342437,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}