{"id":"W2418798131","doi":"10.4014/jmb.1409.09031","title":"Cloning, Expression, and Characterization of a Cold-Adapted and Surfactant-Stable Alginate Lyase from Marine Bacterium Agarivorans sp. L11","year":2015,"lang":"en","type":"article","venue":"Journal of Microbiology and Biotechnology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Education and Child Care","funders":"Ocean University of China; National Natural Science Foundation of China","keywords":"Lyase; Escherichia coli; Enzyme; Biochemistry; Molecular cloning; Cloning (programming); Recombinant DNA; Pulmonary surfactant; Bacteria; Biology; Chemistry; Gene; Gene expression; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000233596,0.0004143734,0.0004151024,0.0002796065,0.0001528485,0.000511485,0.0002677791,0.0003602487,0.0002487596],"category_scores_gemma":[0.0003404599,0.0001393953,0.0004007026,0.0004499438,0.0001914799,0.0003751372,0.0003327181,0.0004966169,0.0003049079],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004132208,"about_ca_system_score_gemma":0.0004091637,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001464358,"about_ca_topic_score_gemma":0.001092966,"domain_scores_codex":[0.9997696,0.00003748621,0.00003755684,0.00004018884,0.0000772528,0.00003795457],"domain_scores_gemma":[0.9997234,0.00004292229,0.00007468375,0.00002981713,0.00007858389,0.0000506029],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003549845,0.00002504779,0.0006038479,0.00004687177,0.000003917024,0.00007351642,0.00002339092,0.00008157106,0.9978945,0.00002570029,0.00001199692,0.001174197],"study_design_scores_gemma":[0.000009638588,0.0001863763,0.007675581,0.000008063355,0.00002774687,0.000467593,0.0001196385,0.001133667,0.9882509,0.00003558066,0.002072838,0.00001230406],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9926274,0.001285778,0.004996237,0.0002132224,0.00002058303,0.00003304691,0.0004508187,0.00002874834,0.0003441397],"genre_scores_gemma":[0.9833828,0.00127861,0.01015665,0.00007682614,0.00001568381,0.00003445071,0.002720116,0.00002947574,0.002305367],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001464358,"threshold_uncertainty_score":0.002998173,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007061709923244742,"score_gpt":0.1996561867475479,"score_spread":0.1925944768243032,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}