{"id":"W2418798131","doi":"10.4014/jmb.1409.09031","title":"Cloning, Expression, and Characterization of a Cold-Adapted and Surfactant-Stable Alginate Lyase from Marine Bacterium Agarivorans sp. L11","year":2015,"lang":"en","type":"article","venue":"Journal of Microbiology and Biotechnology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":44,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Education and Child Care","funders":"Ocean University of China; National Natural Science Foundation of China","keywords":"Lyase; Escherichia coli; Enzyme; Biochemistry; Molecular cloning; Cloning (programming); Recombinant DNA; Pulmonary surfactant; Bacteria; Biology; Chemistry; Gene; Gene expression; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002088319,0.000132237,0.0002804843,0.00009168489,0.00004354556,0.00001071276,0.00008323148,0.000457604,0.00001174965],"category_scores_gemma":[0.00006936988,0.0001108889,0.00002412337,0.00006595299,0.0002592678,0.00001667666,0.0001488538,0.0001637068,4.05822e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006639554,"about_ca_system_score_gemma":0.00004341752,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009028271,"about_ca_topic_score_gemma":0.000007568288,"domain_scores_codex":[0.9992027,0.00007858471,0.0003398134,0.0002177204,0.00002897514,0.0001321581],"domain_scores_gemma":[0.9992079,0.000008675933,0.0004139019,0.0001281748,0.0001739962,0.00006733525],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003992358,0.00004619678,0.006282457,0.00001511347,0.00004973488,0.000004762308,0.00006285703,3.307067e-7,0.9922804,0.00004942996,0.000122309,0.000687202],"study_design_scores_gemma":[0.001057996,0.0005249355,0.005724404,0.00002698487,0.00003085805,0.0003468284,0.00006705621,0.000007180372,0.9532882,0.00003764247,0.03877874,0.0001091619],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964306,0.0008841321,0.0009335903,0.001380245,0.0001919162,0.00007732442,0.00008702737,0.000008107594,0.000007025337],"genre_scores_gemma":[0.9955687,0.002800294,0.001047184,0.0001257384,0.00007702114,9.184375e-7,0.0002517496,0.00001050806,0.000117887],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03899216,"threshold_uncertainty_score":0.4521916,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007061709923244742,"score_gpt":0.1996561867475479,"score_spread":0.1925944768243032,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}