{"id":"W2419265830","doi":"10.1007/978-1-59745-522-0_27","title":"Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification","year":2008,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Centre for Addiction and Mental Health","funders":"","keywords":"Sodium bisulfite; Bisulfite sequencing; DNA methylation; Bisulfite; Methylated DNA immunoprecipitation; Illumina Methylation Assay; genomic DNA; DNA; Molecular biology; Biology; Primer (cosmetics); Epigenomics; Methylation; Computational biology; Chemistry; Genetics; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005143554,0.0006438369,0.0005322937,0.0005597864,0.0002316946,0.0004663408,0.0004975783,0.0004968262,0.00096167],"category_scores_gemma":[0.0005286681,0.0004376635,0.0003979863,0.0004893279,0.0002706677,0.0003642944,0.0003716009,0.0009096373,0.001051654],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002561847,"about_ca_system_score_gemma":0.0004249891,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005578721,"about_ca_topic_score_gemma":0.00245476,"domain_scores_codex":[0.9995522,0.00007010254,0.00002219282,0.0001076299,0.0002138689,0.00003397407],"domain_scores_gemma":[0.99977,0.00009882107,0.00003870298,0.00002750544,0.00004816852,0.00001683539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001074281,0.000008733389,0.000100554,0.00004662236,0.000005524888,0.000008833294,0.00001908982,0.0001346563,0.9934104,0.00007260677,0.00003961134,0.006142502],"study_design_scores_gemma":[0.000002596619,0.00005268056,0.001209823,0.000006125438,0.0000108721,0.00005620286,0.00001484895,0.00128522,0.9936072,0.000126202,0.00362032,0.000007884429],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3365399,0.006226373,0.6493084,0.0003439059,0.00009380057,0.0004865773,0.001690804,0.001540447,0.003769794],"genre_scores_gemma":[0.3242176,0.01205453,0.6487929,0.0001902604,0.00002870082,0.000585093,0.003770214,0.0003405481,0.01002025],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00096167,"threshold_uncertainty_score":0.003217101,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03023264474905739,"score_gpt":0.3183120331101684,"score_spread":0.288079388361111,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}