{"id":"W2419474713","doi":"10.4315/0362-028x-70.9.2133","title":"A Unique Restriction Site in the flaA Gene Allows Rapid Differentiation of Group I and Group II Clostridium botulinum Strains by PCR–Restriction Fragment Length Polymorphism Analysis","year":2007,"lang":"en","type":"article","venue":"Journal of Food Protection","topic":"Botulinum Toxin and Related Neurological Disorders","field":"Medicine","cited_by":2,"is_retracted":false,"has_abstract":false,"ca_institutions":"Health Canada","funders":"Government of Canada","keywords":"Biology; Restriction fragment length polymorphism; Restriction enzyme; Clostridium botulinum; Amplicon; Restriction fragment; Restriction site; Ethidium bromide; Gene; Agarose gel electrophoresis; Genetics; Terminal restriction fragment length polymorphism; Microbiology; Restriction map; Molecular biology; Restriction digest; Clostridiaceae; Polymerase chain reaction; Plasmid; DNA; Toxin","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004893846,0.0006797868,0.0005879993,0.0009550774,0.0004109359,0.000606688,0.0004866769,0.0009367722,0.001718073],"category_scores_gemma":[0.001044647,0.0006416842,0.0008210537,0.0003815765,0.0006122102,0.000428391,0.000484196,0.001360971,0.001165451],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003020957,"about_ca_system_score_gemma":0.0003974932,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001119382,"about_ca_topic_score_gemma":0.001886134,"domain_scores_codex":[0.9993609,0.00008894043,0.00006427336,0.0002288762,0.00009266131,0.0001643668],"domain_scores_gemma":[0.9990971,0.0003795576,0.0001947337,0.000106053,0.00009651438,0.0001260749],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001381108,0.00006140946,0.0004683426,0.0000240734,0.00000657628,0.00005553393,0.00003032504,0.00002903886,0.9954528,0.00009586073,0.00007169299,0.0035663],"study_design_scores_gemma":[0.000089145,0.0005228504,0.0203804,0.00005964422,0.0001033956,0.001691149,0.00007938366,0.001076364,0.9690212,0.0002632254,0.006674427,0.00003885064],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8523057,0.001479959,0.1360627,0.0007288848,0.0003412735,0.0005655403,0.002613954,0.0009717157,0.004930255],"genre_scores_gemma":[0.8367598,0.000829523,0.1479755,0.0006185426,0.0001101894,0.0003722467,0.008896945,0.0002650706,0.004172266],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001718073,"threshold_uncertainty_score":0.005747557,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01512069569443972,"score_gpt":0.231065534175223,"score_spread":0.2159448384807832,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}