{"id":"W2428966220","doi":"10.1007/978-1-4939-2842-2_7","title":"Observing and Quantifying Fluorescent Reporters","year":2015,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Genetics, Aging, and Longevity in Model Organisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Fluorescence; Fluorescent protein; Fluorescence microscope; Transparency (behavior); Fluorescent labelling; Biology; Microscope; Biophysics; Green fluorescent protein; Computer science; Optics; Biochemistry; Gene; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001188579,0.0008427728,0.0006351912,0.0008901216,0.0006083006,0.001176526,0.001209692,0.0009807891,0.002475241],"category_scores_gemma":[0.001197663,0.0005300774,0.0004629269,0.0008042587,0.0007211074,0.00101907,0.0009884415,0.00215707,0.001586668],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008791152,"about_ca_system_score_gemma":0.0007999243,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001061116,"about_ca_topic_score_gemma":0.001310314,"domain_scores_codex":[0.999141,0.0000566477,0.0000560519,0.0002505356,0.0003642443,0.0001314626],"domain_scores_gemma":[0.9993698,0.0001935033,0.00009568911,0.0001061752,0.0001611544,0.00007370731],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001388174,0.00001232898,0.0001483999,0.0000565919,0.000002117921,0.00001950777,0.00003654775,0.0001865658,0.9921824,0.001340275,0.0002411363,0.005760251],"study_design_scores_gemma":[0.000006679049,0.00006472547,0.0007715405,0.00002750337,0.00001101297,0.0001119999,0.00003204822,0.001807848,0.9805456,0.0006401867,0.01596501,0.00001589895],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2126615,0.0037177,0.7606357,0.0006257155,0.0004277186,0.0007218302,0.00175966,0.004757267,0.01469298],"genre_scores_gemma":[0.2738117,0.008184391,0.69635,0.0005110203,0.0001273341,0.001243971,0.00262208,0.0009919641,0.01615749],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002475241,"threshold_uncertainty_score":0.008280456,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08173638524769676,"score_gpt":0.4039121727368113,"score_spread":0.3221757874891145,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}