{"id":"W2432353451","doi":"10.1021/acs.analchem.5b03618","title":"Time-Resolved Nucleic Acid Hybridization Beacons Utilizing Unimolecular and Toehold-Mediated Strand Displacement Designs","year":2015,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"U.S. Naval Research Laboratory; Natural Sciences and Engineering Research Council of Canada; Canada Research Chairs; Defense Threat Reduction Agency; Michael Smith Health Research BC; Office of Naval Research; Canada Foundation for Innovation","keywords":"Molecular beacon; Förster resonance energy transfer; Chemistry; Oligonucleotide; Nucleic acid; Fluorescence; Biophysics; Locked nucleic acid; Luminescence; DNA; Biochemistry; Optoelectronics; Optics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001403174,0.0008293161,0.0005883407,0.0005838041,0.0002788462,0.0009849215,0.001582555,0.001195296,0.001949013],"category_scores_gemma":[0.001614749,0.0007698144,0.0003998213,0.0004197367,0.0007888487,0.001117973,0.0006936385,0.001306761,0.001349693],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009507454,"about_ca_system_score_gemma":0.0005264161,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003100371,"about_ca_topic_score_gemma":0.0004818754,"domain_scores_codex":[0.9985669,0.000268406,0.00009936345,0.0004285573,0.00049657,0.0001400878],"domain_scores_gemma":[0.999231,0.0002461266,0.000221721,0.0001023704,0.0001216195,0.00007720581],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001131151,0.00006620269,0.0001054999,0.0002240841,0.00001198995,0.00005270322,0.0001129471,0.001024807,0.9649538,0.007796318,0.0002037558,0.0253349],"study_design_scores_gemma":[0.00002738409,0.0002799495,0.00008396874,0.00001151842,0.000007801288,0.0001371744,0.00001504416,0.005386925,0.9833106,0.0007884907,0.009915822,0.00003528909],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1848117,0.007344855,0.7948775,0.0005093407,0.0005251432,0.0006938031,0.0002970753,0.002431885,0.008508711],"genre_scores_gemma":[0.3670029,0.00340346,0.6099311,0.0005773227,0.0001052914,0.00109871,0.0004392003,0.0002064166,0.01723565],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.001949013,"threshold_uncertainty_score":0.007420778,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02277008374669218,"score_gpt":0.2751752732490541,"score_spread":0.2524051895023619,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}