{"id":"W2438003748","doi":"10.1007/978-1-60761-588-0_19","title":"Transfection of siRNAs in Multiple Myeloma Cell Lines","year":2010,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"Kay Kendall Leukaemia Fund","keywords":"Small interfering RNA; Transfection; RNA interference; Gene silencing; Streptolysin; Trans-acting siRNA; Cell culture; Cell biology; Functional genomics; Biology; Molecular biology; Gene; Chemistry; RNA; Computational biology; Genomics; Genetics; Genome; Bacterial protein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001175303,0.0007664911,0.001225375,0.0008971991,0.0007471986,0.0008632179,0.001275748,0.0009228552,0.005971082],"category_scores_gemma":[0.0004762413,0.0005666034,0.0007213376,0.001387807,0.0004719585,0.0003910657,0.0006227315,0.00247417,0.006033826],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007928881,"about_ca_system_score_gemma":0.0006299543,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001598864,"about_ca_topic_score_gemma":0.002717339,"domain_scores_codex":[0.9984248,0.0002559577,0.0003073124,0.0003050099,0.0005167461,0.0001902051],"domain_scores_gemma":[0.999575,0.0001058746,0.0000514914,0.0001139198,0.0001057384,0.00004812396],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009571887,0.00007125451,0.00008367863,0.0001137901,0.00001694526,0.00008899876,0.0001000532,0.0002112172,0.9948436,0.001105537,0.0009140204,0.002355244],"study_design_scores_gemma":[0.00005706996,0.0001600573,0.0006234634,0.000011323,0.00003528342,0.0001709802,0.00003263756,0.002014114,0.974519,0.0001822011,0.02218082,0.00001304944],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.4992098,0.005715466,0.3975107,0.001638974,0.001910081,0.006175209,0.04299732,0.006413992,0.03842838],"genre_scores_gemma":[0.6062101,0.006931277,0.2252043,0.0009710363,0.0002273759,0.008474466,0.05956246,0.001473165,0.09094586],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.005971082,"threshold_uncertainty_score":0.01997524,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01615049534678582,"score_gpt":0.3471396162468635,"score_spread":0.3309891209000777,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}