{"id":"W2438760595","doi":"10.1007/978-1-59745-028-7_16","title":"Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins","year":2008,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Hospital for Sick Children","funders":"","keywords":"Proteomics; Organelle; Gel electrophoresis; Membrane protein; Biochemistry; Context (archaeology); Polyacrylamide gel electrophoresis; Membrane; Mitochondrion; Cell biology; Biology; Function (biology); Chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004204534,0.0005464259,0.0006467082,0.0006758284,0.0004428717,0.0005494274,0.00042078,0.0003546412,0.0005923531],"category_scores_gemma":[0.0003643618,0.0002181368,0.0004760469,0.0006775984,0.0002443604,0.0003914459,0.0004676429,0.0007474558,0.001045342],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003177798,"about_ca_system_score_gemma":0.0003827638,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000684413,"about_ca_topic_score_gemma":0.000927127,"domain_scores_codex":[0.999718,0.00003829502,0.00004002414,0.00005906007,0.00009703829,0.00004753111],"domain_scores_gemma":[0.9998438,0.00002757945,0.00002134706,0.00002842416,0.00005349053,0.0000253318],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003943749,0.00002180826,0.0001480253,0.00007302342,0.000009651384,0.0000826239,0.00002240037,0.0000422223,0.9979854,0.000100269,0.00006933236,0.00140588],"study_design_scores_gemma":[0.0000182701,0.0001843032,0.008521872,0.00002426015,0.00005772442,0.001320973,0.0001054798,0.001356159,0.9748317,0.0002715357,0.01328933,0.00001844953],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.896305,0.01273515,0.07674556,0.001257711,0.0002651809,0.0004969388,0.006467081,0.0004700811,0.005257326],"genre_scores_gemma":[0.7835233,0.02004762,0.1503538,0.0008170551,0.0002097915,0.0005901039,0.02999792,0.0004771088,0.01398329],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.000684413,"threshold_uncertainty_score":0.002305686,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01776500880883556,"score_gpt":0.3195895850272609,"score_spread":0.3018245762184253,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}