{"id":"W2442994112","doi":"10.1007/978-1-4939-3378-5_7","title":"Nuclear RNA Isolation and Sequencing","year":2015,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Cancer-related molecular mechanisms research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Polyadenylation; Biology; RNA; Transcriptome; Genetics; DNA sequencing; Deep sequencing; Gene; Post-transcriptional regulation; Computational biology; Gene expression; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002679436,0.004881793,0.002980646,0.004727371,0.005106985,0.002036599,0.003865082,0.001637633,0.04242585],"category_scores_gemma":[0.003792877,0.00247617,0.003775469,0.003141318,0.002914255,0.001052268,0.002274578,0.00693689,0.04796399],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00131511,"about_ca_system_score_gemma":0.004605419,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003608782,"about_ca_topic_score_gemma":0.009419773,"domain_scores_codex":[0.9965612,0.0006919332,0.0003806215,0.001221805,0.000640648,0.0005037658],"domain_scores_gemma":[0.9956964,0.000975474,0.0001913877,0.001906981,0.0008831805,0.0003465393],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006627951,0.0003078382,0.0008886391,0.001593646,0.0001697578,0.0004699482,0.00076805,0.001207589,0.8974382,0.01232673,0.0242856,0.05988118],"study_design_scores_gemma":[0.0004527326,0.0007654076,0.003725847,0.0005471287,0.000552927,0.0008721199,0.000332776,0.006870413,0.5674758,0.01252128,0.4056904,0.0001932165],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02150342,0.002140023,0.8695824,0.001063431,0.00118254,0.008552634,0.04796883,0.01347448,0.03453231],"genre_scores_gemma":[0.04178925,0.003903425,0.7143472,0.002181071,0.0007288389,0.01694066,0.1393362,0.008292006,0.07248132],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.04242585,"threshold_uncertainty_score":0.1419286,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04146395960723569,"score_gpt":0.4055419495210956,"score_spread":0.3640779899138599,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}