{"id":"W2462531281","doi":"10.1007/978-1-62703-694-8_15","title":"Isolation of Zebrafish Gonads for RNA Isolation","year":2013,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Chromosomal and Genetic Variations","field":"Agricultural and Biological Sciences","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Princess Margaret Cancer Centre; Ontario Institute for Cancer Research","funders":"","keywords":"Piwi-interacting RNA; Zebrafish; Biology; Germline; Germ plasm; Gonad; Isolation (microbiology); Germ cell; Genetics; RNA; Evolutionary biology; Bioinformatics; Embryo; Anatomy; RNA interference; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005397715,0.001165951,0.0006570585,0.001780563,0.001261412,0.0003894954,0.0007704657,0.0007186339,0.01480682],"category_scores_gemma":[0.0004287284,0.0008893895,0.000913804,0.0005365952,0.0006087393,0.0004745715,0.00094281,0.002278797,0.007660232],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006648654,"about_ca_system_score_gemma":0.001156857,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004906325,"about_ca_topic_score_gemma":0.01890052,"domain_scores_codex":[0.999625,0.00001601947,0.00003743199,0.0001031748,0.0001567563,0.00006169667],"domain_scores_gemma":[0.9996905,0.00003726326,0.00003922041,0.00005768937,0.0001051242,0.00007018798],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00005150604,0.00001917928,0.0002617691,0.0001477177,0.00001390395,0.0002338131,0.00007258779,0.000068625,0.9939097,0.0007521626,0.0007892183,0.003679774],"study_design_scores_gemma":[0.0003131321,0.0006195785,0.03211778,0.0002951139,0.0001663708,0.001580472,0.000188167,0.00165979,0.7843812,0.001164147,0.1773966,0.0001176422],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3404533,0.01248589,0.3945631,0.001773669,0.002448046,0.02560889,0.1068946,0.01112807,0.1046444],"genre_scores_gemma":[0.3011675,0.00926981,0.4704683,0.00180324,0.0003622117,0.01626675,0.07926258,0.003895155,0.1175045],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01480682,"threshold_uncertainty_score":0.04953372,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02559932006333641,"score_gpt":0.3293500654367044,"score_spread":0.3037507453733679,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}