{"id":"W2466683540","doi":"10.1073/pnas.1603980113","title":"Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study","year":2016,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Advanced Electron Microscopy Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":62,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto","funders":"Canadian Institutes of Health Research; Canadian HIV Trials Network, Canadian Institutes of Health Research; Canada Research Chairs; Government of Canada; Yale University","keywords":"Thermoplasma acidophilum; ATP hydrolysis; Proteasome; Cryo-electron microscopy; Chemistry; Biophysics; ATPase; Biochemistry; AAA proteins; Crystallography; Biology; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006501905,0.00007743747,0.00008448996,0.00002201766,0.0002565868,0.000008714258,0.0008485679,0.00005850119,0.000002960466],"category_scores_gemma":[0.0001595023,0.00003395413,0.00006521189,0.0003201117,0.0004502822,0.00001873975,0.0002234626,0.00006818423,1.769616e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001727145,"about_ca_system_score_gemma":0.00002727911,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000003778491,"about_ca_topic_score_gemma":2.498637e-7,"domain_scores_codex":[0.9990509,0.000009280802,0.0002254973,0.0001892357,0.0004014453,0.0001236282],"domain_scores_gemma":[0.999373,0.00002968032,0.0003761649,0.00002518016,0.0001816156,0.00001437888],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000100696,0.00005500692,0.0004938258,0.000004866303,0.000009422206,2.364022e-10,0.00003203757,0.000003095319,0.9710996,0.02685095,0.001246772,0.0001943947],"study_design_scores_gemma":[0.0001589547,0.0001232419,0.001226227,0.00002575217,0.00001083315,0.000001331518,0.0001310073,0.00004431337,0.9690295,0.02867521,0.0005255128,0.0000481291],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952747,0.0001128683,0.0003318469,0.003556859,0.000009045109,0.0004239705,0.00002486914,0.000006028868,0.000259787],"genre_scores_gemma":[0.9989854,0.00005209822,0.0004865235,0.000164709,0.00002320458,0.00003642875,1.947431e-7,0.000003911995,0.0002475054],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003710699,"threshold_uncertainty_score":0.1973484,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01633450541676995,"score_gpt":0.320556737452787,"score_spread":0.304222232036017,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}