{"id":"W2468401125","doi":"10.1038/srep27697","title":"Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels","year":2016,"lang":"en","type":"article","venue":"Scientific Reports","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":79,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ottawa Hospital; University of Ottawa","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Transactivation; Inducer; Gene; Gene expression; Biology; Regulation of gene expression; Transcription (linguistics); Activator (genetics); Genetics; Transcription factor; Cell biology; Computational biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003450395,0.0007028654,0.0004930067,0.0002669162,0.0001577299,0.0004969873,0.0005028805,0.0004905975,0.001042033],"category_scores_gemma":[0.0004951806,0.0002149228,0.0004189097,0.000211048,0.0003926543,0.0003580079,0.0004132436,0.001026656,0.0007108242],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003304431,"about_ca_system_score_gemma":0.0003311675,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004205241,"about_ca_topic_score_gemma":0.0008564803,"domain_scores_codex":[0.9996375,0.00004693371,0.00006201646,0.00008270673,0.0001312925,0.00003962934],"domain_scores_gemma":[0.9995475,0.00009442663,0.0001971552,0.00006559056,0.00005374534,0.00004153133],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001251638,0.00001131703,0.00004408027,0.00002178753,0.000003431203,0.00002210414,0.00000620533,0.00007154245,0.9985057,0.00008838249,0.00001435233,0.001198653],"study_design_scores_gemma":[0.000004062942,0.00005207137,0.0002695023,0.000003042884,0.00001037495,0.0002450099,0.000005445078,0.0005575098,0.9971029,0.00002418439,0.001721193,0.000004800144],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8807325,0.001925857,0.1117514,0.0003962267,0.000122574,0.0001428801,0.0003893128,0.001067643,0.003471632],"genre_scores_gemma":[0.9520903,0.001403805,0.04174841,0.0001129997,0.00002205768,0.00007119532,0.0005710422,0.0003116994,0.003668526],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001042033,"threshold_uncertainty_score":0.003485978,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01413873292588399,"score_gpt":0.2621867563522327,"score_spread":0.2480480234263487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}