{"id":"W2512802152","doi":"10.1093/protein/gzw043","title":"Isolation of TGF-β-neutralizing single-domain antibodies of predetermined epitope specificity using next-generation DNA sequencing","year":2016,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph; Wilfrid Laurier University; University of Ottawa; National Research Council Canada","funders":"","keywords":"Epitope; Panning (audio); Phage display; Antibody; Molecular biology; Epitope mapping; Biology; Computational biology; Single-domain antibody; Ectodomain; Receptor; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003235192,0.0001330313,0.0002571225,0.0002036165,0.00006211995,0.00001503335,0.00003251303,0.0000792287,0.0000129642],"category_scores_gemma":[0.00009915061,0.000100678,0.00005155696,0.0001893828,0.00004630211,0.0001968066,0.00001616952,0.00007924419,5.170948e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001029742,"about_ca_system_score_gemma":0.00005104279,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007451173,"about_ca_topic_score_gemma":0.000002871027,"domain_scores_codex":[0.9990313,0.00006365724,0.0003210368,0.0001896879,0.0002029057,0.000191396],"domain_scores_gemma":[0.9995514,0.00006837262,0.0001064897,0.00006853745,0.0001453247,0.00005987909],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002017752,0.00003145102,0.0009800447,0.0002422279,0.0000359828,0.000002101316,0.0001393392,0.001454498,0.9933574,0.0002013701,0.000002978268,0.003350879],"study_design_scores_gemma":[0.000335445,0.0004022616,0.000990129,0.0003887133,0.00001935951,0.00003990276,0.000009978228,0.154859,0.8427705,0.00006525809,0.00002427922,0.00009519169],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.653977,0.0001616346,0.3454035,0.00005353333,0.00003062665,0.0003348286,0.000001955426,0.00002464882,0.00001225373],"genre_scores_gemma":[0.920976,0.0000680212,0.07867649,0.000002927008,0.0001636564,0.00000887291,0.000003389634,0.00001693041,0.00008371119],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2669989,"threshold_uncertainty_score":0.4105529,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.109595895598213,"score_gpt":0.2769870438968428,"score_spread":0.1673911482986297,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}