{"id":"W2514965956","doi":"10.3389/fmicb.2016.01344","title":"Construction of a Multiplex Promoter Reporter Platform to Monitor Staphylococcus aureus Virulence Gene Expression and the Identification of Usnic Acid as a Potent Suppressor of psm Gene Expression","year":2016,"lang":"en","type":"article","venue":"Frontiers in Microbiology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Health and Medical Research Fund; University of Hong Kong","keywords":"Virulence; Staphylococcus aureus; Plasmid; Reporter gene; Microbiology; Biology; Gene; Repressor; Gene expression; Ampicillin; Virulence factor; Promoter; Bacteria; Antibiotics; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005738805,0.0007652263,0.0006468124,0.0006413794,0.0001897809,0.0004504747,0.0004124673,0.000459941,0.0009913646],"category_scores_gemma":[0.0002651762,0.0002824603,0.0005021687,0.0005436401,0.0002609309,0.000437094,0.0005249227,0.0008341701,0.000462698],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002513585,"about_ca_system_score_gemma":0.0003403396,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002533012,"about_ca_topic_score_gemma":0.0005125844,"domain_scores_codex":[0.9995939,0.00007845272,0.0000339753,0.00008968549,0.0001512672,0.00005275251],"domain_scores_gemma":[0.9997726,0.00004753742,0.00006841479,0.00003177656,0.00003671747,0.00004299738],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002819111,0.00002445277,0.00006460165,0.00002032103,0.000002212205,0.00001312204,0.000008758705,0.00004094935,0.9989931,0.00005473925,0.00001358983,0.0007358893],"study_design_scores_gemma":[0.000005586756,0.0002115234,0.0004827277,0.000003822385,0.000008705371,0.00007975622,0.00001110443,0.000854042,0.9973791,0.00002920759,0.0009291856,0.000005308],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8591291,0.001500824,0.1346228,0.0002588762,0.0001130051,0.0004255965,0.001780337,0.0006065114,0.001562948],"genre_scores_gemma":[0.8017089,0.001417279,0.1869822,0.00008695689,0.00002974932,0.0006208353,0.003201524,0.00009910702,0.005853499],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009913646,"threshold_uncertainty_score":0.003316462,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008691440236011747,"score_gpt":0.2322648481386033,"score_spread":0.2235734079025916,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}