{"id":"W2523838195","doi":"10.21769/bioprotoc.1738","title":"RAB21 Activity Assay Using GST-fused APPL1","year":2016,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Sherbrooke","funders":"National Institute of General Medical Sciences","keywords":"Rab; GTPase; Effector; Guanine nucleotide exchange factor; GTP'; Transport protein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002434527,0.0001741946,0.0001315225,0.00003361495,0.00008527011,0.00002295759,0.0001515546,0.0002228677,0.0002080773],"category_scores_gemma":[0.00002209367,0.0001243908,0.0001241364,0.00006734856,0.00007634985,0.000006741916,0.00004450613,0.00005477272,0.00003505566],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002561816,"about_ca_system_score_gemma":0.00007921686,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003170856,"about_ca_topic_score_gemma":0.0000190004,"domain_scores_codex":[0.9989767,0.00006803687,0.0001672873,0.0003777186,0.0001528899,0.0002573341],"domain_scores_gemma":[0.9993465,0.000007653151,0.00008881065,0.0004129885,0.00005460746,0.00008941722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003179692,0.00007103114,0.003190882,0.00002841639,0.00002315616,0.000003701217,0.000005726278,0.000001561295,0.9853443,0.00002621555,0.0001019842,0.01088507],"study_design_scores_gemma":[0.001417631,0.0001075312,0.001372641,0.00001914902,0.000008516554,0.00000377659,0.000002696248,0.00004358941,0.6120651,0.00002659947,0.3847554,0.0001773565],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"protocol","genre_gemma":"protocol","genre_scores_codex":[0.2970782,0.000006734209,0.2007288,0.0001824436,0.0001572367,0.4994117,0.00004150992,0.00007507744,0.00231826],"genre_scores_gemma":[0.4507209,0.000001634665,0.0007250754,0.0000780066,0.0005289136,0.5465816,0.0001142547,0.00004759345,0.001202071],"genre_candidate":"protocol","genre_consensus":"protocol","teacher_disagreement_score":0.3846534,"threshold_uncertainty_score":0.507251,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02464972874758621,"score_gpt":0.2944660219351166,"score_spread":0.2698162931875304,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}