{"id":"W2524357818","doi":"10.21769/bioprotoc.889","title":"Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration","year":2013,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Plant nutrient uptake and metabolism","field":"Agricultural and Biological Sciences","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Pi; Biochemistry; Phosphatase; Intracellular; ATP hydrolysis; Phosphate; Metabolism; Hydrolysis; Acid phosphatase; Enzyme; Compartment (ship); Mitochondrion; Isozyme; Respiration; Extraction (chemistry); Cytosol; Biology; Chemistry; Chromatography; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001514601,0.004387822,0.002622472,0.003934183,0.001208188,0.0009833309,0.002039062,0.00115828,0.003792029],"category_scores_gemma":[0.001062031,0.00117634,0.001349667,0.003768538,0.0009400948,0.001210029,0.001211002,0.004098658,0.004176388],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001089651,"about_ca_system_score_gemma":0.001443977,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001734644,"about_ca_topic_score_gemma":0.002836127,"domain_scores_codex":[0.9976501,0.0002340283,0.000302289,0.0006114489,0.0008669026,0.0003352693],"domain_scores_gemma":[0.9987121,0.0002466528,0.0001446348,0.0002095362,0.0005677519,0.0001193701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001047893,0.0001126639,0.0002130502,0.0002543062,0.00003726752,0.0001185714,0.00004631322,0.0001197067,0.9937458,0.0001934704,0.0003220918,0.004731825],"study_design_scores_gemma":[0.00009102774,0.0007017743,0.008116849,0.0001472968,0.0002064826,0.0004928848,0.00009458313,0.003332493,0.9396083,0.00129612,0.04583568,0.00007656873],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2369845,0.04191424,0.6271339,0.001328911,0.001887423,0.01271582,0.034051,0.007897123,0.036087],"genre_scores_gemma":[0.1652516,0.03707691,0.6291575,0.001141551,0.0009458327,0.01754019,0.1005599,0.00166773,0.04665887],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004387822,"threshold_uncertainty_score":0.01268554,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02220451396251847,"score_gpt":0.2589418946704179,"score_spread":0.2367373807078995,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}