{"id":"W2524357818","doi":"10.21769/bioprotoc.889","title":"Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration","year":2013,"lang":"en","type":"article","venue":"BIO-PROTOCOL","topic":"Plant nutrient uptake and metabolism","field":"Agricultural and Biological Sciences","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Pi; Biochemistry; Phosphatase; Intracellular; ATP hydrolysis; Phosphate; Metabolism; Hydrolysis; Acid phosphatase; Enzyme; Compartment (ship); Mitochondrion; Isozyme; Respiration; Extraction (chemistry); Cytosol; Biology; Chemistry; Chromatography; Botany","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00019526,0.0001264209,0.0001495101,0.000009648106,0.0001305698,0.00006984475,0.00009681629,0.0001114916,0.0003393672],"category_scores_gemma":[0.00005379872,0.00005426634,0.00003771473,0.0001453162,0.00005673251,0.0005758618,0.00003111608,0.00007964081,0.00002608928],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000141613,"about_ca_system_score_gemma":0.00001467341,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002960938,"about_ca_topic_score_gemma":0.0000481415,"domain_scores_codex":[0.9990776,0.0000806401,0.0002130605,0.0002351396,0.0001981417,0.0001953733],"domain_scores_gemma":[0.9994878,0.0000250393,0.0002311075,0.00005725733,0.0001119254,0.00008686322],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006526626,0.0001167204,0.0004265934,0.00002134782,0.000001856465,6.248024e-7,0.000009003094,1.569069e-8,0.7746846,0.0002407863,0.0002552483,0.2241779],"study_design_scores_gemma":[0.0004844484,0.0002007147,0.02879395,0.00003858987,0.000003866824,0.000002683353,0.00001775196,0.0001054908,0.8561298,0.0003526215,0.1137456,0.0001244091],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"protocol","genre_scores_codex":[0.6069442,0.00001161945,0.00005309546,0.0002660052,0.00002971584,0.3923395,0.00003555225,0.00004459792,0.000275721],"genre_scores_gemma":[0.428887,0.000001443965,0.0003432742,0.00002079372,0.00008358992,0.5703006,0.0000262968,8.703147e-7,0.0003361102],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.2240535,"threshold_uncertainty_score":0.3715833,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02220451396251847,"score_gpt":0.2589418946704179,"score_spread":0.2367373807078995,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}