{"id":"W2528435521","doi":"10.1007/978-1-4939-6451-2_13","title":"Conditional Toxin Splicing Using a Split Intein System","year":2016,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Victoria","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Intein; RNA splicing; Protein splicing; Toxin; Chemistry; Protein engineering; FKBP; Computational biology; Biology; Biochemistry; Gene; RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005436916,0.0005732381,0.0003216242,0.0003934642,0.0002953885,0.0006252793,0.000551039,0.000553498,0.001425821],"category_scores_gemma":[0.0002463892,0.0004330864,0.0005666469,0.0003004396,0.000686683,0.0004988016,0.0009092643,0.001769465,0.0009088668],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004300812,"about_ca_system_score_gemma":0.0003150946,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002527517,"about_ca_topic_score_gemma":0.0004838339,"domain_scores_codex":[0.9995502,0.00007253422,0.00005535559,0.00009823094,0.000155057,0.00006862106],"domain_scores_gemma":[0.9995779,0.0001009842,0.0001067688,0.0001192733,0.00003096193,0.00006409963],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005710408,0.00001844797,0.00007408276,0.00001434751,0.000004443334,0.00003557046,0.00001388587,0.00006632628,0.9980171,0.0007159266,0.00003659753,0.0009461739],"study_design_scores_gemma":[0.00001043508,0.00003406798,0.0003864075,0.000002677667,0.000009116052,0.0001510731,0.000007887352,0.0009708434,0.9970915,0.0001378302,0.001192582,0.000005381354],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.8930568,0.0004535268,0.1018102,0.0002577376,0.0001267014,0.0001565677,0.000691746,0.0006894609,0.00275734],"genre_scores_gemma":[0.9557458,0.0004267013,0.03585047,0.0001071465,0.00002007948,0.0001001391,0.001363667,0.0003712533,0.006014828],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001425821,"threshold_uncertainty_score":0.004769862,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02387952340539387,"score_gpt":0.3521660983583079,"score_spread":0.328286574952914,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}