{"id":"W2531773509","doi":"10.1093/nar/gkw914","title":"Altered stoichiometry<i>Escherichia coli</i>Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation","year":2016,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Skolkovo Institute of Science and Technology; National Institute of General Medical Sciences; Russian Science Foundation; Ministry of Education and Science of the Russian Federation; Russian Foundation for Basic Research; M.J. Murdock Charitable Trust; U.S. Department of Energy","keywords":"Trans-activating crRNA; CRISPR; Biology; Effector; CRISPR interference; RNA; Escherichia coli; RNA interference; Genetics; Random hexamer; Cell biology; Cas9; Molecular biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001641302,0.0003787894,0.0002120523,0.000153002,0.0001224883,0.0004617742,0.0002402311,0.0002658559,0.001098372],"category_scores_gemma":[0.0003471867,0.0002342877,0.0002230471,0.00009275992,0.0001849557,0.0002268426,0.0002736807,0.0004413229,0.0003772833],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004347266,"about_ca_system_score_gemma":0.0001464377,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003497289,"about_ca_topic_score_gemma":0.0004403206,"domain_scores_codex":[0.9997042,0.00003777626,0.00003182597,0.0000633791,0.0001042388,0.00005857429],"domain_scores_gemma":[0.9996973,0.00003827524,0.0001249963,0.00005146161,0.00002581001,0.00006212958],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003059386,0.000011077,0.0001990051,0.000005085132,0.000002418938,0.00001937641,0.000004145981,0.00007358457,0.9992244,0.0001003158,0.00001468036,0.0003152514],"study_design_scores_gemma":[0.000005749826,0.0001085199,0.002765637,0.000001366357,0.000006809561,0.0001827304,0.000007278895,0.001102188,0.9950804,0.00004287164,0.0006912283,0.000005330576],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9946727,0.0001375974,0.003720001,0.00002474954,0.00001355842,0.00001505677,0.00008166471,0.0001295133,0.001205352],"genre_scores_gemma":[0.9956607,0.00004289026,0.002843652,0.0000278154,0.000003171423,0.00001035702,0.0002126567,0.00004589673,0.00115272],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001098372,"threshold_uncertainty_score":0.003674388,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03488729887169334,"score_gpt":0.3347871699295171,"score_spread":0.2998998710578237,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}