{"id":"W2531773509","doi":"10.1093/nar/gkw914","title":"Altered stoichiometry<i>Escherichia coli</i>Cascade complexes with shortened CRISPR RNA spacers are capable of interference and primed adaptation","year":2016,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; Skolkovo Institute of Science and Technology; National Institute of General Medical Sciences; Russian Science Foundation; Ministry of Education and Science of the Russian Federation; Russian Foundation for Basic Research; M.J. Murdock Charitable Trust; U.S. Department of Energy","keywords":"Trans-activating crRNA; CRISPR; Biology; Effector; CRISPR interference; RNA; Escherichia coli; RNA interference; Genetics; Random hexamer; Cell biology; Cas9; Molecular biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003600967,0.0001450248,0.0001929102,0.0001489507,0.00008130942,0.00002687164,0.0002141994,0.0001094101,0.00002996575],"category_scores_gemma":[0.0001457001,0.0001040296,0.00003233027,0.0002639813,0.0002939327,0.000007578089,0.0001531157,0.0001380857,0.00000350302],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000027019,"about_ca_system_score_gemma":0.00008379607,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008927253,"about_ca_topic_score_gemma":0.0001815188,"domain_scores_codex":[0.9986542,0.0000921047,0.0001808269,0.0003632477,0.0003132093,0.0003964232],"domain_scores_gemma":[0.9991511,0.00004991275,0.0000555229,0.0003434235,0.0002525602,0.0001474684],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002641553,0.00003339703,0.003478335,0.00005506602,0.00005777844,0.000001902011,0.0001864675,0.00005341621,0.9870547,0.00003190427,0.000430182,0.008352685],"study_design_scores_gemma":[0.0009409579,0.0008371554,0.03010686,0.0001220563,0.00001273268,0.000006904433,0.0009409561,0.0005803591,0.9629283,0.00003403522,0.003300079,0.0001896328],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9635674,0.0006345912,0.0348203,0.0002228233,0.00003769344,0.000245264,0.00002457866,0.00001292291,0.0004344087],"genre_scores_gemma":[0.9951364,0.0003909212,0.00385976,0.0000221847,0.00007193985,0.00002286317,0.00001031854,0.00003089874,0.0004547346],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03156897,"threshold_uncertainty_score":0.4242204,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03488729887169334,"score_gpt":0.3347871699295171,"score_spread":0.2998998710578237,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}