{"id":"W2537882088","doi":"10.1261/rna.056226.116","title":"Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers","year":2016,"lang":"en","type":"article","venue":"RNA","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":67,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba; Simon Fraser University","funders":"Natural Sciences and Engineering Research Council of Canada; Cancer Research Society","keywords":"RNA; Spinach; Aptamer; Fluorophore; Biology; Riboswitch; Biochemistry; Biophysics; Ligand (biochemistry); Binding site; Small molecule; Non-coding RNA; Fluorescence; Molecular biology; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008648122,0.00008988352,0.0001068537,0.00002693126,0.00006938348,0.000008670234,0.00006572737,0.00007143588,0.000001140802],"category_scores_gemma":[0.00004254611,0.0000501727,0.00003909126,0.00007192027,0.0002381299,0.000003356618,0.00009092107,0.00002737263,2.254103e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008151019,"about_ca_system_score_gemma":0.00001028974,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007782135,"about_ca_topic_score_gemma":0.00001323345,"domain_scores_codex":[0.9994787,0.00002753615,0.0001229567,0.0001936549,0.00007293782,0.0001042334],"domain_scores_gemma":[0.9996287,0.000008369209,0.00009020872,0.0001938791,0.00004696381,0.00003182754],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001614283,0.000008281089,0.001510038,0.00001172079,0.00001861383,2.642048e-7,0.00001643709,1.273595e-7,0.9757295,0.00003448574,0.0001715401,0.02248288],"study_design_scores_gemma":[0.0001541579,0.00008545125,0.003791293,0.00004169463,0.00002362224,0.000005311992,0.00006181902,0.00001938487,0.9933392,0.00008587579,0.002304549,0.00008758248],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9982819,0.000237698,0.0008028634,0.0003203021,0.00002404064,0.00008336263,0.00001377103,0.00001004647,0.000226004],"genre_scores_gemma":[0.9977031,0.0008589799,0.001072242,0.00005521496,0.00003864607,0.000001330745,0.000008104,0.000009196091,0.0002531802],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02239529,"threshold_uncertainty_score":0.2045984,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01282136232653678,"score_gpt":0.2565403371945004,"score_spread":0.2437189748679636,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}