{"id":"W2542138991","doi":"10.1002/cbic.201600492","title":"A Tandem Green–Red Heterodimeric Fluorescent Protein with High FRET Efficiency","year":2016,"lang":"en","type":"article","venue":"ChemBioChem","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"University of Alberta","keywords":"Förster resonance energy transfer; Fluorescence; Green fluorescent protein; Protein engineering; Tandem; Stokes shift; Intramolecular force; Biophysics; Nile red; Fluorescent protein; Chemistry; Fusion protein; Biology; Recombinant DNA; Biochemistry; Gene; Stereochemistry; Materials science","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004441364,0.0003123897,0.0004250467,0.0001849356,0.0002810503,0.0003852463,0.0003895439,0.0004918138,0.0008956779],"category_scores_gemma":[0.0002107673,0.0002345349,0.0002937583,0.0002870595,0.0002276738,0.0002908594,0.0004091431,0.0006701512,0.001204464],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003875993,"about_ca_system_score_gemma":0.0002384601,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003656712,"about_ca_topic_score_gemma":0.0005194707,"domain_scores_codex":[0.9996588,0.00005359847,0.00002692516,0.00009817184,0.0001263563,0.0000361175],"domain_scores_gemma":[0.999904,0.00001137801,0.00001572904,0.00002212956,0.00001890976,0.00002778667],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001684816,0.00001622882,0.00007098511,0.00001359018,0.000002347286,0.00002809276,0.000009713934,0.0001090132,0.9975455,0.0001899126,0.0001003936,0.001897215],"study_design_scores_gemma":[0.00001055077,0.00008115239,0.0006957749,0.00000281169,0.000007576165,0.0005529478,0.00001429427,0.002459101,0.9900753,0.00005600816,0.006035809,0.000008721801],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.895013,0.0009040465,0.09540879,0.0003358394,0.00007997535,0.000130234,0.0006817577,0.001007636,0.006438613],"genre_scores_gemma":[0.8893385,0.0007296856,0.0981786,0.0001334258,0.00001386904,0.0001116542,0.00126522,0.0002726584,0.009956418],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008956779,"threshold_uncertainty_score":0.002996325,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004801087065609854,"score_gpt":0.2209091640590183,"score_spread":0.2161080769934085,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}