{"id":"W2546444580","doi":"10.1101/pdb.prot090035","title":"Combining the Dihydrofolate Reductase Protein-Fragment Complementation Assay with Gene Deletions to Establish Genotype-to-Phenotype Maps of Protein Complexes and Interaction Networks","year":2016,"lang":"en","type":"article","venue":"Cold Spring Harbor Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; PROTEO","funders":"","keywords":"Protein-fragment complementation assay; Dihydrofolate reductase; Biology; Complementation; Genetics; Gene; Ploidy; Phenotype; Protein–protein interaction; Fusion protein; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001191554,0.001074686,0.0008613408,0.002980103,0.0006653585,0.0009776689,0.001144589,0.0006511171,0.001846007],"category_scores_gemma":[0.0009044756,0.0006915066,0.001122034,0.001422785,0.0009080917,0.0006145624,0.001267466,0.001928024,0.001207475],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001121276,"about_ca_system_score_gemma":0.000669023,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002787664,"about_ca_topic_score_gemma":0.006068413,"domain_scores_codex":[0.998934,0.0001982601,0.0001044253,0.0003201915,0.000344427,0.00009870171],"domain_scores_gemma":[0.9980823,0.0008154367,0.0003764693,0.0003622432,0.0001944207,0.0001692308],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004269888,0.00004544024,0.000351448,0.00004341785,0.00002571431,0.00003038156,0.00002057449,0.0002555937,0.9959757,0.0003574999,0.00006351051,0.002788037],"study_design_scores_gemma":[0.000019888,0.0001268128,0.008116901,0.000009570766,0.0000419176,0.0002334934,0.00002449845,0.004921356,0.9830092,0.0005853949,0.002881927,0.00002892101],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3441455,0.001682001,0.6395405,0.0004019038,0.0001377493,0.0007722835,0.004747832,0.003038311,0.00553395],"genre_scores_gemma":[0.5702246,0.003114101,0.4107862,0.0004339051,0.00004147051,0.001488681,0.006580254,0.00130514,0.006025636],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002980103,"threshold_uncertainty_score":0.008135498,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01888488366232408,"score_gpt":0.2888058172182578,"score_spread":0.2699209335559337,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}